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Analytical and Bioanalytical Chemistry Research، جلد ۱۰، شماره ۱، صفحات ۱۵-۲۳
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عنوان انگلیسی Disposable Paper-based Biosensing Platform for Procalcitonin Detection
چکیده انگلیسی مقاله Procalcitonin (PCT), acts a significant role in the screening of bacterial infection (BI), which is one of the world’s leading health problems. This work presents a simple, efficient, and convenient glass fiber paper (GFP) substrate-based innovative biosensor applications for BI-specific PCT biomarker detection. To enhance the conductivity of the GFP substrate, a self-accumulating single layer of AuNP is used on the GFP surface. This monolayer of AuNP in GFP provides a platform for antibody immobilization and shows high electrochemical conductivity. Further, the AuNP/GFP electrode was modified with antibodies specific to PCT followed by the addition of bovine serum albumin (BSA) to block unspecific binding sites for PCT detection. The PCT/BSA/ab/AuNP/GFP bioelectrode has been successfully used to determine PCT in a serum sample with amended stability. Scanning electronic microscopy, transmission electron spectroscopy, Fourier transform infrared, UV-Vis spectroscopy, and X-ray diffraction were used to examine the structural and morphological properties. Using an electrochemical impedance response study, the developed PCT/BSA/ab/AuNP/GFP bioelectrode showed the lowest detection limit of 100 pg/ml concerning PCT detection in the linear range of 107-102 pg/ml. Electrochemical response studies show that the developed BSA/ab/AuNP/GFP bioelectrode has a lifespan of 30 days and can be used to assess PCT in the 107-102 pg/ml ranges. Moreover, this evaluated sensor offers many merits, including economical, small sample volume, single-use, and fast analysis over conventional methods, making the platform an alternative tool for PCT screening. This developed bioelectrode also shows good reproducibility in an acceptable range.
کلیدواژه‌های انگلیسی مقاله electrochemical impedance spectroscopy,Gold Nanoparticles,Glass fiber paper monoclonal antibody,procalcitonin
نویسندگان مقاله Yachana Gupta |
Applied Science Department, The NorthCap University, Gurugram, India

Aditya Sharma Ghrera |
Applied Science Department, The NorthCap University, Gurugram, India

نشانی اینترنتی https://www.analchemres.org/article_155025_688b34784df0ec902238d65d16bf51d1.pdf
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