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Iranian Journal of Biotechnology، جلد ۲۱، شماره ۳، صفحات ۱-۱۳
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عنوان انگلیسی The Effect of Collagen and Fibrin Hydrogels Encapsulated with Adipose Tissue Mesenchymal Stem Cell-Derived Exosomes for Treatment of Spinal Cord Injury in a Rat Model
چکیده انگلیسی مقاله Background: Mesenchymal stem cell (MSC) derived exosomes (MSC-DE) have been demonstrated to be potential candidates for the treatment of rat spinal cord injury (SCI).
Objective: The effect of AD-MSC and AD-MSC-DE encapsulated into collagen and fibrin hydrogels on the treatment of SCI in a rat animal model was investigated for introducing a new effective SCI treatment method
Materials and Methods: The AD-MSC-DE was isolated using ultra-centrifugation at 100,000×g for 120 min and characterized by different methods. Fibrin and collagen hydrogels were synthesized and then mixed with AD-MSC-DE suspension. the characterized AD-MSC-DE were encapsulated into collagen and fibrin hydrogels. eighteen adult male Wister rats were randomly classified into 3 equal groups (n=6): the control group (SCI rat without treatment), SCI rat treated with either AD-MSC-DE encapsulated in collagen hydrogel or encapsulated in fibrin hydrogel groups. the treatment approaches were evaluated using clinical, histological, and molecular assays.
Results: The AD-MSC-DE encapsulated into fibrin and collagen groups showed better clinical function than the control group. The AD-MSC-DE encapsulated into fibrin and collagen also improved SCI-induced polio and leuko-myelomalacia and leads to higher expression of NF protein than the control group. In the AD-MSC-DE encapsulated into collagen and fibrin leads to up-regulation the mean levels of NEFL (23.82 and 24.33, respectively), eNOS (24.31 and 24.53, respectively), and CK19 mRNAs (24.23 and 23.98, respectively) compared to the control group.
Conclusion: The AD-MSC-DE encapsulated within ECM-based hydrogel scaffolds such as collagen and fibrin can regenerate the injured nerve in SCI rats and reduce spinal cord lesion-induced central neuropathic pain.
کلیدواژه‌های انگلیسی مقاله Adipose Mesenchymal Stem Cell-Derived Exosomes,collagen,Fibrin,Hydrogel,Spinal cord injury
نویسندگان مقاله Zohreh Afsartala |
Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Science, Tehran, Iran

Mahmoudreza Hadjighassem |
Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Science, Tehran, Iran

Sadegh Shirian |
Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

Somaye Ebrahimi-Barough |
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Leyla Gholami |
Department of Periodontics, Dental Research Center, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran

Gilda Parasmanesh |
Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Ziba Malekshahi |
Department of Medical Biotechnology School of Advanced Technologies in Medical, Tehran University of Medical Sciences

Latifeh Karimzadeh |
Department of Animal Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran

Jafar Ai |
Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Science, Tehran, Iran

نشانی اینترنتی https://www.ijbiotech.com/article_171478_4ebcf424e37e5d16753e9e4d6243eb29.pdf
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