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Iranian Journal of Biotechnology، جلد ۲۱، شماره ۳، صفحات ۲۴-۳۲
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عنوان انگلیسی In vitro Gluten Degradation Using Recombinant Eurygaster Integriceps Prolyl Endoprotease: Implications for Celiac Disease
چکیده انگلیسی مقاله Background: Celiac disease (CD) is a gluten-sensitive chronic autoimmune enteropathy. A strict life-long gluten-free diet
is the only efficient and accepted treatment until now. However, maintaining a truly gluten-free status is both difficult and costly, often resulting in a social burden for the person. Moreover, 2 to 5 percent of patients fail to improve clinically and histologically upon elimination of dietary gluten. Therefore, novel therapeutic approaches, including gluten degrading enzymes, are an unmet need of celiac patients.
Objectives: To evaluate the function of sunn pest prolyl endoprotease for gluten and gliadin hydrolysis in vitro.
Materials and Methods: The spPEP was expressed as a recombinant protein in E. coli BL21 (DE3), and its catalytic
activity was assessed by SDS-PAGE and RP-HPLC analyses.
Results: Production of a 100-kDa spPEP protein was confirmed by SDS-PAGE and western blot analysis. Also, we
demonstrate that spPEP efficiently degrades gluten and α-gliadin (30-40 kDa) in vitro under conditions similar to the GI
and is resistant to pepsin and trypsin.
Conclusion: The gathered data demonstrated that spPEP might be a novel candidate for Oral Enzymatic Therapy (OET)
in CD and other gluten-related disorders.
کلیدواژه‌های انگلیسی مقاله autoimmunity,celiac disease,Gluten,Prolyl endoprotease,Prolamin
نویسندگان مقاله Effat Noori |
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mojgan Bandehpour |
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mohammad Reza Zali |
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Bahram Kazemi |
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

نشانی اینترنتی https://www.ijbiotech.com/article_171692_53a834ca459d66de8e4394053169f264.pdf
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