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Iranian Journal of Biotechnology، جلد ۲۱، شماره ۳، صفحات ۳۳-۴۳
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عنوان انگلیسی MiRNA-145-5p Restrains Malignant Behaviors of Breast Cancer Cells Via Downregulating H2AFX Expression
چکیده انگلیسی مقاله Background: Breast cancer is a prevalent tumor with high aggressiveness among female populations. MiRNA-145-5p
plays an important role in multiple cancers.
Materials and Methods: qRT-PCR detected miRNA-145-5p and histone protein family member X (H2AFX) mRNA
expression in breast cancer cells, and western blot determined the protein expression of H2AFX. After predicting the target genes via the bioinformatics methods, the targeting relationship between miRNA-145-5p and H2AFX was verified by dualluciferase, RIP, and RNA pull-down assays. The relationship between H2AFX and clinical indexes was also analyzed. Furthermore, the effects of miRNA-145-5p/H2AFX regulatory axis on breast cancer cell progression were determined by colony formation, wound healing, CCK-8, and Transwell assays.
Results: The results suggested that miRNA-145-5p was markedly lowly-expressed in breast cancer tissue and cells,
while H2AFX was upregulated, which had a positive correlation with T stages of breast cancer. Besides, overexpressed
miRNA-145-5p was found to remarkably suppress progression of breast cancer cells. As bioinformatic analysis predicted
that H2AFX was the potential target of miRNA-145-5p, the dual-luciferase assay was conducted, which demonstrated
that miRNA-145-5p negatively regulated the expression of H2AFX by targeting its 3’-UTR. The rescue experiment
demonstrated that overexpression of miRNA-145-5p could offset the promotion effects of oe-H2AFX on malignant
progression.
Objective: Our study is aimed at exploring how miRNA-145-5p functions in breast cancer cells.
Conclusion: Our findings confirmed that miRNA-145-5p hindered malignant progression of breast cancer by negatively
regulating H2AFX. MiRNA-145-5p/H2AFX axis may be a novel therapeutic target for breast cancer.
کلیدواژه‌های انگلیسی مقاله breast cancer,H2AFX,Invasion,miRNA-145-5p,migration,Proliferation
نویسندگان مقاله Deshun Yao |
Department of Breast Surgery, Tangshan People’s Hospital, China

Xin Chen |
Breast center, Municipal Hospital Affiliated to Taizhou University, China

نشانی اینترنتی https://www.ijbiotech.com/article_171693_6c9c05160e8eb21f08806888dbe3d4b7.pdf
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