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Iranian Journal of Biotechnology، جلد ۲۱، شماره ۳، صفحات ۴۴-۵۲
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عنوان انگلیسی SOX2 Overlapping Transcript (SOX2-OT) Enhances the Lung Cancer Malignancy Through Interaction with miR-194-5p/SOX5 Axis
چکیده انگلیسی مقاله Background: Lung cancer is one of the most common types of cancer and a leading cause of cancer-related deaths
worldwide. Therefore, it is useful to know the biomarkers involved in the malignancy of lung cancer.
Objectives: This study aimed to show that SOX2-OT as a long non-coding RNA (IncRNA) regulates gene expression via
the SOX2-OT/miR-194-5p/SOX5 axis molecular pathway in lung cancer.
Materials and Methods: A549 cells transfected with siRNA-SOX2-OT and the expression of SOX2-OT and miR-194-5p
genes were analyzed by real-time PCR before and after transfection. In addition, the expression of the B-catenin, MMP9,
phosphorylated and activated STAT3 (p-STAT3), SOX5, and VEGF proteins before and after transfection was investigated
by Western blotting.
Results: After using siRNA-SOX2-OT, an increase in the expression of miR-194-5p and a decrease in the expression of
B-catenin, SOX5, p-STAT3 activated STAT3, VEGF, and MMP9 proteins was observed.
Conclusions: According to the results of the present study, an increase in SOX2-OT in lung cancer seems to stimulate the
expression of beta-catenin, SOX5, MMP9, and VEGF thus support the malignancy of lung cancer cells.
کلیدواژه‌های انگلیسی مقاله β-catenin,miRNA-194-5P,MMP9,P-STAT3,SOX5,SOX2-OT,VEGF
نویسندگان مقاله Zahra Sadeghi |
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Fatemeh Dodangheh |
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Jamshid Raheb |
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

نشانی اینترنتی https://www.ijbiotech.com/article_171696_67ffc7020885fc88ef130a400c61238e.pdf
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