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Iranian Journal of Biotechnology، جلد ۲۱، شماره ۳، صفحات ۷۸-۸۸
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عنوان انگلیسی Anti-Inflammatory Properties of Extract of the Hairy Root of Native Portulaca Oleracea L. and Its Effect on Some Inflammatory Genes Expression in Rat Microglial Cells
چکیده انگلیسی مقاله Background: Most herbs play significant roles in the treatment of various diseases. Because dopamine functions in the
anti-inflammatory process and the presence of this substance in Portulaca Oleracea L. native plant, investigating this
plant’s anti-inflammatory properties in treating neurological diseases is interesting.
Objectives: The objective of this study was to estimate the NO production and the expression level of inflammatory genes
in lipopolysaccharide (LPS)-treated microglial cells affected by P. oleracea L. extraction.
Materials and Methods: P. oleracea L. hairy root extract was isolated, and the primary microglial cell of the rat was
isolated from glial cells and confirmed by immunocytochemistry analysis. Microglial cells were pretreated with different
concentrations of P. oleracea L. extract and then treated with 1 μg.mL-1 LPS. The control group did not receive any
treatment. The NO level in culture supernatants was measured by the Griess method. The mRNA expression levels of
iNOS (inducible Nitric oxide synthase) and TNF-α (tumor necrosis factor-alpha) in LPS-treated microglial cells were
evaluated using Real-Time PCR.
Results: The present study determined that 0.1 mg. mL-1 of the P. oleracea L. extract decreased the NO production in rat
microglial cells. Different concentrations of the P. oleracea L. extract had no prominent effects on LPS-treated cell viability. The results of real-time PCR indicated that P. oleracea L extracts suppressed the mRNA expression levels of iNOS and TNF-α in LPS-treated cells. MTT assay determined that P. oleracea L. extract was not cytotoxic, and the anti-inflammatory P. oleracea L. extract effects observed were not because of cell death.
Conclusion: P. oleracea L. extract might be helpful as an anti-inflammatory agent in treating inflammatory diseases.
کلیدواژه‌های انگلیسی مقاله Inflammation,iNOS,microglia,P. oleracea L. extract,TNF- α
نویسندگان مقاله Somayeh Babashpour |
Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.

Khosro Piri |
Department of Biodiversity & Ecosystems Management Environmental Sciences Research Institute, Shahid Beheshti University, Tehran, Iran.

Farzaneh Sabouni |
Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.

Sevil Babashpour |
Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.

نشانی اینترنتی https://www.ijbiotech.com/article_171687_5e48d93da041550caef0813f952aba31.pdf
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