این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Biotechnology، جلد ۲۰، شماره ۴، صفحات ۱-۱۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Use of Gelatin as a Sacrificial Agent in Combination with Ultrasonication to Improve Cell Infiltration and Osteogenesis of Nanofibrous PCL-nHA Scaffolds for Bone Tissue Engineering
چکیده انگلیسی مقاله Background: Electrospinning has been widely used to prepare nanofibrous scaffolds for bone tissue engineering. However, owing to the small pore size of electrospun scaffolds, cellular infiltration and tissue ingrowth are impossible. The use of sacrificial fibers (like poly ethylene oxide (PEO)) in combination with ultrasonication has been an appropriate way to increase the pore size of the electrospun scaffolds in our previous work. However, it is uneconomical due to the high cost of PEO.
Objectives: In this study, gelatin was chosen as a novel sacrificial agent in co-electrospun with polycaprolacton-nanohydroxyapatite (PCL-nHA).
Materials and Methods: After electrospinnig, gelatin was washed with water, and the prepared scaffold was ultrasonicated. Morphological and structural properties of the prepared scaffolds were studied by SEM. Fourier transform infrared (FTIR) spectroscopy and water contact angle analysis were used to evaluate the removal of gelatin.
Results: According to the SEM results, the pore size of the modified scaffolds was increased 3-folds compared to the control sample. For PCL-nHA: gelatin (80:20) after the treatment, the average cell infiltration was 42.7 μm, while there was no infiltration for the control group. The modified electrospun scaffold significantly enhanced the osteogenic differentiation of hBMSCs as verified by increased ALP activity and upregulation of runt-related transcription factor 2 (RUNX2), collagen type 1 (COL1) and osteocalcin (OCN) genes.
Conclusion: Co-electrospun PCL-nHA with gelatin as a sacrificial agent in combination with ultrasonication may be an effective, economic and controllable method to increase the pore size in electrospun scaffolds for bone tissue engineering applications.
کلیدواژه‌های انگلیسی مقاله Electrospinning,Gelatin,Osteogenic differentiation,Polycaprolacton-nanohydroxyapatite (PCL-nHA)
نویسندگان مقاله Saeed Moghadam Deymeh |
Biomedical Engineering Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran

Sameereh Hashemi-Najafabadi |
Biomedical Engineering Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran

Mohamadreza Baghaban-Eslaminejad |
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

Fatemeh Bagheri |
Biotechnology Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran

نشانی اینترنتی https://www.ijbiotech.com/article_157963_8f4a03b0dcb33d4d5b389a061204e972.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات