این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Biotechnology، جلد ۱۹، شماره ۳، صفحات ۷۰-۷۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Comparative Transcriptomic Analysis on White and Blue Flowers of Platycodon grandiflorus to Elucidate Genes Involved in the Biosynthesis of Anthocyanins
چکیده انگلیسی مقاله Background: Platycodon grandiflorus has long been used in Northeast Asia as a food and folk medicine to treat various diseases. The intense blue color of P. grandiflorus corolla is its characteristic feature.
Objectives: By comparing deep transcriptomic data of P. grandiflorus and its white cultivar, we intended to elucidate the molecular mechanisms concerning the biosynthesis of anthocyanins in this plant.
Material and Methods: We sampled blue mature flowers (PgB) and yellow young buds (PgY) of P. grandiflorus. Meanwhile, mature flowers (PgW) of P. grandiflorus white cultivar were also collected for RNA extraction and next-generation sequencing. After high-throughput sequencing, Trinity software was applied for de novo assembly and the resultant 49934 unigenes were subjected for expression analysis and annotation against NR, KEGG, UniProt, and Pfam databases.
Results: In all, 32.77 Gb raw data were generated and the gene expression profile for the flowers of P. grandiflorus was constructed. Pathway enrichment analysis demonstrated that genes involved in flavone and flavonol biosynthesis were differently expressed.
Conclusions: The extremely low expression of flavonoid-3’,5’-hydroxylase in PgY and PgW was regarded as the reason for the formation of its white cultivar. Our findings provided useful information for further studies into the biosynthetic mechanism of anthocyanins.
 
کلیدواژه‌های انگلیسی مقاله Anthocyanin,biosynthetic pathway,Flavonoid-3’,5’-hydroxylase,next-generation sequencing,Platycodon grandifloras
نویسندگان مقاله Yanping Lv |
School of Pharmacy, Anhui University of Chinese Medicine; Hefei 230012, China

Xiaohui Tong |
School of Life Sciences, Anhui University of Chinese Medicine; Hefei 230012, China

Pengfei Zhang |
School of Pharmacy, Anhui University of Chinese Medicine; Hefei 230012, China

Nianjun Yu |
School of Pharmacy, Anhui University of Chinese Medicine; Hefei 230012, China

Shuangying Gui |
School of Pharmacy, Anhui University of Chinese Medicine; Hefei 230012, China

Rongchun Han |
School of Pharmacy, Anhui University of Chinese Medicine; Hefei 230012, China.

Dezhu Ge |
Department of Research and Development, Anhui Jiren Pharmaceutical Company; Bozhou 236800, China

نشانی اینترنتی https://www.ijbiotech.com/article_135045_ddf5b5d68eca7e79c236629d062d4f66.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات