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Iranian Journal of Biotechnology، جلد ۱۹، شماره ۲، صفحات ۱۹-۲۹

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عنوان انگلیسی Estimation of Personal Environment Via Fingertip Microbiome and Mobile Phone Surfaces
چکیده انگلیسی مقاله Background: Fingerprints can serve to identify individuals, but fingerprint quality may be deteriorated, even to the point of eliminating fingerprints, due to the external environment. Objective: Poor fingerprint quality cannot be effectively used to identify individuals; hence, the need for other methods. Materials and Methods: We investigated the utility of bacterial communities and the only microorganisms present in the sample to identify internal and external factors in individuals. Samples included eight participants’ fingerprints and their mobile phone surfaces. Bacterial DNA in the samples was sequenced using next-generation sequencing to target the V3– V4 region in the 16S ribosomal RNA gene. The QIIME program was used to perform a taxonomic assignment and alpha diversity and beta diversity analyses based on the sequence data. Results: Until now, personal identification has only relied on microbial communities. However, this study identified microbial differences according to Korean mobile phones, fingertips, or gender, and confirmed the possibility of characterization of samples when it was difficult to identify individuals by the microbial community. The biodiversity and composition of individual bacterial communities were affected by internal and external environments. Bacteria from individuals and mobile phones were shared due to contact between mobile phone surfaces and fingertips. Of the eight Koreans, six of the fingertips and mobile phone samples matched each other for personal identification. Conclusions: This study confirmed that the bacteria from an individual could be matched with the contact object and could be used as forensic evidence. Such bacterial profiling of individuals may confer forensic evidence and serve as a basis for improving the accuracy of forensic verification.
کلیدواژه‌های انگلیسی مقاله 16S ribosomal RNA,Bacterial communities,Fingertips,Forensic verification,Internal and External Factors,Mobile Phone

نویسندگان مقاله YeonJeong Ok |
Department of Biomedical Laboratory Science, Eulji University, 77, Gyeryong-ro, 771 beon-gil, Jung-gu, Daejeon, 34824, Republic of Korea

Song Hee Lee |
Department of Senior Healthcare, BK21 plus program, Graduate School, Eulji University, 77, Gyeryong-ro, 771 beon-gil, Jung-gu, Daejeon, 34824, Republic of Korea

Hee Sang You |
Department of Senior Healthcare, BK21 plus program, Graduate School, Eulji University, 77, Gyeryong-ro, 771 beon-gil, Jung-gu, Daejeon, 34824, Republic of Korea

Young Ju Lee |
Department of Biomedical Laboratory Science, Eulji University, 77, Gyeryong-ro, 771 beon-gil, Jung-gu, Daejeon, 34824, Republic of Korea

Sang Sun Kang |
Department of Biology Education, Chungbuk National University, 1, Chundae-ro, Seowon-gu, Cheongju, Chungbuk, 28644, Republic of Korea

Sung Hee Hyun |
Department of Biomedical Laboratory Science, Eulji University, 77, Gyeryong-ro, 771 beon-gil, Jung-gu, Daejeon, 34824, Republic of Korea


نشانی اینترنتی https://www.ijbiotech.com/article_133417_469ff2b14165237a13caeb26f461e97f.pdf
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