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Iranian Journal of Biotechnology، جلد ۱۸، شماره ۴، صفحات ۳۹-۴۶
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عنوان انگلیسی Expression Alteration of Candidate Rice MiRNAs in Response to Sheath Blight Disease
چکیده انگلیسی مقاله Background: MicroRNAs, as small non-coding RNAs, are recently reported to be involved in plant defense system against pathogens including fungi.
Objective: In this research, it was intended to investigate candidate susceptible rice (Oryza Sativa) Osa-miRNA expression alteration following the infection by Rhizoctonia solani.
Materials and Methods: To this aim, literature review suggested eight conserved plant miRNAs that are involved in other plant-pathogen interactions. Then, sixty days old rice plants (Hashemi, susceptible cultivar) were inoculated with R. solani and candidate miRNA expression alterations were investigated 2 hpi (hours post inoculation), 2 dpi (days post inoculation) and 6 dpi.
Results: RT-qPCR analysis suggested four subgroups of candidate miRNAs based on the time of their responses to the pathogenesis of R. solani. While Osa-miR-156 was early-responsive, Osa-miR159 was the last-responsive and Osa-miR167, Osa-miR171, Osa-miR408, and Osa-miR444 were late responsive to R. solani infection. Osa-miR166 and Osa-miR393 were non-responsive to this infection, compared to the mock-inoculated control group. Consistently, Os-SPL3 and Os-MADS known target genes were expressed in reverse correlation to Osa-miR156 and Osa-miR444, respectively.
Conclusions: From these data, it is suggested that both early (Osa-miR-156) and late (Osa-miR167, Osa-miR171, OsamiR408, Osa-miR444) responsive miRNAs might be involved in R. solani infection in rice plants.
کلیدواژه‌های انگلیسی مقاله miRNA,Rhizoctonia solani,Rice plant
نویسندگان مقاله Soheila Talesh Sasani |
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran; Department of Biology, University of Guilan, Rasht, Iran

Bahram M. Soltani |
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Rahim Mehrabi |
Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Hossein Samavatian |
Department of Plant Protection Research, Isfahan Agricultural and Natural Resources Research and Education Center, AREEO, Isfahan, Iran.

Fereidoun Padasht-Dehkaei |
Plant Protection Department, Rice Research Institute of Iran (RRII), Rasht, Iran

نشانی اینترنتی https://www.ijbiotech.com/article_111374_cfccb93c9389b5a1aa4ba955ff03bd5a.pdf
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