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Iranian Journal of Biotechnology، جلد ۱۸، شماره ۳، صفحات ۴۵-۵۴
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عنوان انگلیسی An Integrative In Silico Mathematical Modelling Study of The Anti-Cancer Effect of Clove Extract (Syzygium aromaticum) Combined With In Vitro Metabolomics Study Using 1HNMR Spectroscopy
چکیده انگلیسی مقاله Background: Clove oil is known for its medicinal properties. The mechanism of anti-cancer properties of Syzygium aromaticum were investigated by mathematical modelling on the genome scale with metabolomics using 1H Nuclear Magnetic Resonance spectroscopy on Raji cells.
Objectives: An integrative analysis correlated the metabolites identified by 1HNMR and genes with the detected pathways.
Materials and Methods: Raji cells treated with clove oil were collected and sent for 1HNMR spectroscopy and the spectra analyzed by MATLAB and Human Metabolome Database for metabolite identification. Pathway and topology analysis was implemented using the genes and metabolites in the integrative analysis of Metaboanalyst software.
Results: 50% inhibitory concentration of clove oil was 50 μg/ml and the model anticipated 74 genes with differentiating metabolites being some amino acids, cholesterol and fucose.
Conclusions: The integrative study predicted that the anti cancer mechanism of clove oil involves novel enzymes, as likely drug targets, 24-dehydrocholesterol reductase and 7-dehydrocholesterol reductase in cholesterol biosynthesis, dehydrofolate reductase in one carbon metabolism and serine palmitoyl-transferase long chain in sphingolipid biosynthesis.
 
کلیدواژه‌های انگلیسی مقاله genes and pathways,In silico mathematical model,methanol clove extract,Raji cells
نویسندگان مقاله Mehri Azadi |
Biochemistry Department, Pasteur Institute of Iran, Tehran, Iran.

Fatemeh Alemi |
Chemical Engineering Department, Islamic Azad University, Tehran, Iran.

Sedigheh Sadeghi |
Biochemistry Department, Pasteur Institute of Iran, Tehran, Iran.

Maryam Mohammadi |
Biochemistry Department, Pasteur Institute of Iran, Tehran, Iran

Nowrooz Ali Rahimi |
Biochemistry Department, Pasteur Institute of Iran, Tehran, Iran.

Sako Mirzaie |
Biology Department, Islamic Azad University, Pasdaran Avenue, Sanandaj, Iran.

Farideh Vahabi |
Biochemistry Department, Pasteur Institute of Iran, Tehran, Iran.

Shirin Parvaz |
Biochemistry Department, Pasteur Institute of Iran, Tehran, Iran

Mohammad Arjmand |
Biochemistry Department, Pasteur Institute of Iran, Tehran, Iran

Zahra Zamani |
Biochemistry Department, Pasteur Institute of Iran, Tehran, Iran

نشانی اینترنتی https://www.ijbiotech.com/article_118178_ff4b7986a63469cbff99a5492fb57a90.pdf
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