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Iranian Journal of Biotechnology، جلد ۱۸، شماره ۳، صفحات ۱۰۱-۱۰۶
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کلیدواژه‌های فارسی مقاله
عنوان انگلیسی A Facile Method for Morphological Characterization at Nano Scale
چکیده انگلیسی مقاله Background: Dynamic light scattering (DLS) and electron microscopy (EM) are the most practical techniques for nanoparticles (NPs) characterization. However, the impediments which involved the sample preparation method lead to failure in provided results of mentioned device analysis. These problems will be intensifying, if the examined samples are the soft nanocarriers such as organic ones or biological samples.
Objectives: In order to achieve the appropriate results from DLS and EM analysis, an optimized protocol was introduced by this research which would prepare samples with high degree of quality and accuracy.
Materials and Methods: Morphological analysis of prepared polymeric nanocarriers (micelles, nanogels) by this protocol were done. Filtration, dilution and sonication as three crucial and effectiveness steps of sample preparation were assessed through DLS data and EM images.
Results: This research has tried to introduce a facile method with novelty of simplicity and rapidity. These triple steps could improve the quality of morphological data. The obtained results indicated that sample preparation methods have the most effective factors on sample size distribution and homogeneity of desired samples.
Conclusion: The suggested optimized preparation method will be helpful for all soft nanomaterial’s samples.
کلیدواژه‌های انگلیسی مقاله Dynamic Light Scattering (DLS),EM,Soft nanocarriers,Triple steps
نویسندگان مقاله Ali Dinari |
Polymer Reaction Engineering Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.

Seyedeh Sahar Mortazavi Farsani |
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Soheila Mohammadi |
Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Farhood Najafi |
Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran.

Mahdi Abdollahi |
Polymer Reaction Engineering Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.

نشانی اینترنتی https://www.ijbiotech.com/article_110415_deb35b7903b3d121d6f149d2c141a93a.pdf
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