| چکیده انگلیسی مقاله |
Staphylococcus aureus are Gram positive bacteria known to acquire antibiotic resistance rapidly and pose a major challenge to clinicians worldwide. Infections by methicillin resistant Staphylococcus aureus (MRSA) are usually associated with increased mortality and prolonging of treatment. Samples (n = 706) from diverse sources (livestock, pets, animal handlers, human hospital) were collected and screened for the presence of MRSA by phenotypic and genotypic methods. The incidence of Staphylococcus aureus was greater in goats (42.00%; 28.20 - 56.80%, confidence interval [CI] 95.00%) followed by cattle (13.50%; 9.20 - 18.80%, CI 95.00%), humans (12.90%; 9.30 - 17.40%, CI 95.00%) and dogs (12.90%; 8.10 - 19.20%, CI 95.00%). Significantly higher incidence of MRSA was observed in dogs (65.00%; 40.80 - 84.60%, CI 95.00%), compared to other hosts namely cattle (48.00%; 26.50 - 64.30%, CI 95.00%), humans (35.00%; 20.20 - 52.50%, CI 95.00%) and goats (10.00%; 1.20 - 30.40%, CI 95.00%). All the S. aureus isolates were further screened for thermostable nuclease (nuc gene) by polymerase chain reaction (PCR). The incidence of nuc gene in cattle, dog, goat and human were found to be 3.30% (1.30 - 6.60%, CI 95.00%), 5.20% (2.30 - 9.90%, CI 95.00%), 28.00% (16.20 - 42.50%, CI 95.00%) and 9.10% (6.00 - 13.00%, CI 95.00%), respectively. Comparative evaluation of two PCR primers (mecA-162 and mecA-310) indicated the former one as more rational choice for detection of MRSA. Overall, the results of our study indicated possible risk of zoonotic transmission of MRSA from canines. |
| نویسندگان مقاله |
Dharitree Sonowal |
Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India
Sandeep Ghatak |
Division of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, India
Acheenta Gohain Barua |
Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India
Srinivas Kandhan |
Division of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, India
Razibuddin Ahmed Hazarika |
Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India
Arnab Sen |
Division of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, India
Samir Das |
Division of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, India
Sarat Sonowal |
Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India
Rajeev Kumar Sharma |
Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India
Shantanu Tamuly |
Department of Veterinary Biochemistry, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India
Chimanjita Phukan |
Department of Microbiology, Gauhati Medical College, Srimanta Sankaradeva University of Health Sciences, Guwahati, India
Ajanta Sharma |
Department of Microbiology, Gauhati Medical College, Srimanta Sankaradeva University of Health Sciences, Guwahati, India
Poznur Hussain |
Department of Veterinary Public Health, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, India
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