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Veterinary Research Forum، جلد ۱۴، شماره ۴، صفحات ۲۱۳-۲۱۹
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عنوان انگلیسی Genotypic evaluation of Pasteurella multocida isolated from cattle and sheep by pulsed-field gel electrophoresis
چکیده انگلیسی مقاله Pasteurella multocida a Gram-negative bacterium exists as a commensal in the upper respiratory tracts of livestock, and poultry. It is causative agent of a range of diseases in mammals and birds including fowl cholera in poultry, atrophic rhinitis in pigs and bovine hemorrhagic septicemia in cattle and buffalo. This study aimed to isolate P. multocida from sheep and cattle lungs sampled and assessed by bacteriological procedures and pulse field gel electrophoresis (PFGE) characterization. In this study 52 isolated of P. multocida were obtained (2016-2017) from clinically healthy and diseased animals (sheep and cattle) evaluated by PFGE for determining the relationship between them. According to the results of this study 12 sheep isolates had similarities above 94.00% and two cattle isolates showed similarities above 94.00%. When compared between sheep and cattle, most isolates showed a similarity of less than 50.00% indicating the great differences between isolates. It is noteworthy that in the present study, performed by PFGE to determine the type of P. multocida isolates, a very high distinction was made to determine the type of isolates and the relationship between isolates based on fragments in their genome using enzymes.
کلیدواژه‌های انگلیسی مقاله Pasturella mutocida,Pasteurellosis,PFGE Shiraz
نویسندگان مقاله Mohammad Tabatabaei |
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Ali Janparvar |
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Mohammad Motamedifar |
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

نشانی اینترنتی https://vrf.iranjournals.ir/article_702484_f6893bc46119332a25bc878ad670b76b.pdf
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