این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Veterinary Research Forum، جلد ۱۴، شماره ۱، صفحات ۷-۱۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Rapid and visual detection of an isolated and identified goose parvovirus (GPV) strain by a loop-mediated isothermal amplification assay
چکیده انگلیسی مقاله Gosling plague caused by goose parvovirus (GPV), a highly infectious septic disease with high mortality, has caused substantial loss in the waterfowl industry. A method for the rapid detection of GPV is needed. In this study, we isolated the virus strain of GPV in May 2020 and applied it to the loop-mediated isothermal amplification (LAMP) assay. We designed five sets of primers for the goose parvovirus VP3 gene by LAMP. The GV-1 primer set was selected to detect GPV sensitively and rapidly. LAMP was more sensitive compared to PCR. In addition, the LAMP method could complete detection within 60 min which was faster than the PCR assay. The LAMP provided a convenient and effective experimental method for detection of GPV for inspection and quarantine departments and health care units in China, and it is expected to become a simple and routine detection method, especially suitable for goose farms.
کلیدواژه‌های انگلیسی مقاله goose parvovirus,Gosling plague,Loop-mediated isothermal amplification
نویسندگان مقاله Jun-Ting Liu |
Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China

Yan-Hui Chen |
Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China

Yi-Feng Pei |
Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China

Qian Yu |
Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China

Ruth Afumba |
Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China

Hao Dong |
Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China

نشانی اینترنتی https://vrf.iranjournals.ir/article_697938_99f5f2a00b02dd8c05eb421c25323621.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات