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Veterinary Research Forum، جلد ۱۳، شماره ۳، صفحات ۴۰۳-۴۰۷
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عنوان انگلیسی A novel competitive ELISA for detection of antibodies against bovine viral diarrhea virus infection
چکیده انگلیسی مقاله Diagnosis of bovine viral diarrhea (BVD) relies on the detection of antibodies against its viral causing agent, bovine viral diarrhea virus (BVDV). Here, we designed a novel competitive ELISA (cELISA) using the most immunogenic part of BVDV nonstructural protein 3 (NS3), as a single ELISA recombinant antigen, along with a monoclonal antibody to detect antibodies against BVDV in sera of infected animals. Hence, 197 serum samples were tested by this cELISA and the results were compared to the results obtained from virus neutralization test (VNT) as the gold standard method for diagnosis of BVD. McNemar’s test indicated that there was no significant difference between the results of this newly designed cELISA and VNT. Meanwhile, kappa coefficients showed that there was a high correlation between these two assays. The relative sensitivity and specificity of cELISA with respect to VNT were 93.90% and 100%, respectively, suggesting that this newly designed cELISA could be a useful diagnostic tool for detection of BVDV infection. Moreover, as NS3 is highly conserved among Pestiviruses and the developed ELISA is a competitive one, it could potentially be applied to detect BVDV infection in other domestic and wildlife species.
کلیدواژه‌های انگلیسی مقاله Bovine viral diarrhea,competitive ELISA,Nonstructural Protein 3
نویسندگان مقاله Masoud Reza Seyfi Abad Shapouri |
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Pezhman Mahmoodi |
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran

Masoud Ghorbanpour Najafabadi |
Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

Mohammad Rahim Haji Hajikolaei |
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Parastoo Moradi Choghakabodi |
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Mohsen Lotfi |
Quality Control Department, Razi Vaccine and Serum Research Institute, Agricultural Education and Extension Research Organization (AREEO), Karaj, Iran

Mahdi Pourmahdi Boroujeni |
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Maryam Ekhtelat |
Department of Pharmacognosy, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Maryam Daghari |
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

نشانی اینترنتی https://vrf.iranjournals.ir/article_253001_3602f78c1e6da7bfd4cdfcb186cf1838.pdf
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