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JCR 2016
جستجوی مقالات
یکشنبه 23 آذر 1404
Veterinary Research Forum
، جلد ۱۳، شماره ۲، صفحات ۱۵۵-۱۶۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژههای فارسی مقاله
عنوان انگلیسی
Prevalence of Shiga toxin-producing Escherichia coli and Salmonellae and some associated hematologic and biochemical profile alterations in lambs
چکیده انگلیسی مقاله
Lamb enteritis constitutes an economic burden on sheep production worldwide. We aimed to estimate the prevalence of Shiga toxin-producing
Escherichia coli
(STEC)
and
Salmonellae
among diarrheic lambs at Kafrelsheikh Governorate, Egypt and to detect the associated clinical, hematologic, biochemical, and antioxidant parameters. Fifty diarrheic and twenty apparently healthy control lambs were examined clinically, and hematologically. Diarrheic lambs had a significant elevated body temperature, respiratory and pulse rate, most of hemogram para-meters, total proteins and albumin, oxidative stress markers malonaldiahyde and nitric oxide levels, liver enzymes, urea and creatinine than control group. On the other hand, these diarrheic lambs had significant reduction in total leukocyte count and lymphocytes, antioxidant biomarkers super oxide dismutase activities and reduced glutathione than control lambs.
E. coli
and
Salmonella
spp. were isolated from 32.00%
and 16.00% of diseased lambs, respectively. Serotyping and biochemical tests of examined samples identified 16
E. coli
isolates belonged to 10 different serotypes; O6, O8, O26:H11, O75, O84:H21, O103:H2, O114:H4, O121:H7, O128:H2 and O163:H2. All isolates are STEC as they harbor either Shiga-toxin 1 or Shiga-toxin 2 genes or both. One isolate carries intimin gene (
eae
A) and classified as EHEC; O26:H11. The obtained nine isolates of
Salmonella
carry enterotoxin (
Stn
) genes, eight of them carry hyper-invasive locus (
hilA
) gene, all isolates belonged to six serotypes;
S
.
Enteritidis
,
S. Heidelberg
,
S. Tsevie
,
S. Typhimurium
,
S. Essen
, and
S. Infantis
. Lamb diarrhea was prevalent in the studied area and might constitute a veterinary and public health threat. Alteration in hemato-biochemical para-meters and oxidative–anti-oxidant balance could help adopt appropriate treatment regimens.
کلیدواژههای انگلیسی مقاله
Escherichia coli,Hemato-biochemical profile,Lamb enteritis,Virulence genes,Salmonella
نویسندگان مقاله
Abd Allah Ahmed Mokhbatly |
Department of Clinical Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El Sheikh, Egypt
Nahawand Elsheikh |
Department of Clinical Pathology, Animal Health Research Institute, Kafr El Sheikh Branch, Agriculture Research Center, Giza, Egypt
Emad Wadeed Ghazy |
Department of Clinical Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El Sheikh, Egypt
Adel Mohamed Elgamal |
Unit of Bacteriology, Animal Health Research Institute, Kafr El Sheikh Branch, Agriculture Research Center, Giza, Egypt
Yamen Mohammed Hegazy |
Department of Animal Medicine, Faculty of Veterinary Medicine, Kafr El Sheikh University, Kafr El Sheikh, Egypt
Doaa Hosny Assar |
Department of Clinical Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El Sheikh, Egypt
نشانی اینترنتی
https://vrf.iranjournals.ir/article_251344_d8a32169dd44dd10b49ad2c10afffca2.pdf
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en
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