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Veterinary Research Forum، جلد ۱۳، شماره ۱، صفحات ۵۵-۶۰
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عنوان انگلیسی Detection and distribution of virulence genes in Aeromonas hydrophila ‎isolates causing infection in cultured carps ‎
چکیده انگلیسی مقاله Aeromonas hydrophila is a bacterium associated with many diseases and disorders such as fin rot, skin ulcers and lethal hemorrhagic septicemia in fish. It bears several virulence factors including type III secretion system (T3SS), aerolysin, cytolytic enterotoxin and enzymes (e.g. hemolysins, lipase) that seem to play an important role in its pathogenesis. Detection of virulence markers by polymerase chain reaction (PCR) is a key procedure in defining the pathogenic ability of pathogenic bacteria and preparing a vaccine for its treatment. In this sense, this study was aimed to determine the frequency of virulence genes in isolates obtained from infected cultured carps in Khuzestan province. Out of 200 moribund carps with septicemic symptoms, 125 isolates were belonged to the motile aeromonads and 59 isolates were identified as A. hydrophila by biochemical methods. Finally, using PCR analysis, 31 isolates were identified as A. hydrophila. Five virulence genes were detected in these isolates including hemolysin, aerolysin, cytolytic enterotoxin and T3SS (aopB and ascV) by specific primers. Results showed that 23 (74.19%), 18 (58.06%), 16 (51.61%), 13 (41.63%) and 10 (32.25%) isolates possessed cytolytic enterotoxin, hemolysin, aerolysin, and T3SS genes, respectively. The results of the present study showed that among 31 isolates, only five isolates had all of dominant virulence genes. Thirteen other isolates had genotypes including hlyA+, aerA+, and act+. The remaining isolates had at least one virulence gene. This study showed that determination of the virulence genes by PCR can be a reliable method to identify a potential pathogenic Aeromonad strain.
کلیدواژه‌های انگلیسی مقاله Aeromonas hydrophila,‎ Carp,‎ Hemorrhagic septicemia,‎ Virulence genes
نویسندگان مقاله Mina Ahangarzadeh |
South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension ‎Organization (AREEO), Ahvaz, Iran‎

Masoud Ghorbanpour Najafabadi |
Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

Rahim Peyghan |
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran‎

Hossein Houshmand |
South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension ‎Organization (AREEO), Ahvaz, Iran‎

Mostafa Sharif Rohani |
Department of Fish diseases, Iranian Fisheries Research Science Institute (IFSRI), Agriculture Research, Education and Extension Organization (AREEO), Tehran, Iran

Mehdi Soltani |
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

نشانی اینترنتی https://vrf.iranjournals.ir/article_248038_ab1fe1ee3311f0d82e2bf740facc7422.pdf
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