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Veterinary Research Forum، جلد ۱۲، شماره ۴، صفحات ۴۰۱-۴۰۷
عنوان فارسی
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عنوان انگلیسی Characterization of a novel bullet-shaped lytic bacteriophage against extensively drug-resistant Pseudomonas aeruginosa isolated from human and domestic sources
چکیده انگلیسی مقاله Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates of P. aeruginosa were collected from May to September 2018. Wastewater samples were used for isolation of lytic phage against XDR P. aeruginosa isolates. Host range, thermal and pH stability, adsorption rate, latent period, burst size and morphology of phage were determined following the standard protocols. Morphological characteristics of the phage revealed that it belonged to Podoviridae family and it was named vB-PaeP-007. Although the phage had a narrow host range, 47 out of 70 XDR isolates were susceptible to it. The adsorption rate, latent period and burst size of vB-PaeP-007 were approximately 89.80% in 8 min, 10 min and 93 phages per cell, respectively. Its lysis activity remained at a wide range of pH (4 up to 12) and temperature (– 20.00 up to 70.00 ˚C). Regarding the physiological features and host range of the vB-PaeP-007 phage, it could be a promising candidate for phage therapy and bio-controlling of infections from XDR isolates of P. aeruginosa in human and livestock storage centers.
کلیدواژه‌های انگلیسی مقاله Bacteriophage,Extensively Drug Resistant,Phage therapy,Pseudomonas aeruginosa
نویسندگان مقاله Mohadeseh Barazandeh |
State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, China

Khashayar Shahin |
State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, China

Abolghasem Hedayatkhah |
Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research and Utrecht University, Den Burg, The Netherlands

Majid Komijani |
Department of Biology, Faculty of Science, Arak University, Arak, Iran

Mojtaba Mansoorianfar |
Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran

نشانی اینترنتی https://vrf.iranjournals.ir/article_246168_8eb589ce1ddce9c2af78baa6e579f273.pdf
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