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Veterinary Research Forum، جلد ۱۲، شماره ۴، صفحات ۴۴۵-۴۵۰
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عنوان انگلیسی Introducing a new anti-Rhipicephalus (Boophilus) microplus tick recombinant vaccine candidate using cathepsin and tropomyosin multi-epitope gene
چکیده انگلیسی مقاله Rhipicephalus (Boophilus) spp. are important vectors for Babesia and Anaplasma species causing severe economic losses in livestock. Chemical compounds are commonly used to control tick infestation; however, acaricides resistance in tick has led to move toward alternative strategies such as vaccination. In this study, we introduced a vaccine candidate, namely CaTro against Rh. microplus tick composing of immunogenic B-cell epitopes derived from Rh. microplus cathepsin L and tropomyosin proteins. To evaluate this vaccine candidate, firstly the CaTro sequence was inserted into the prokaryotic expression vector and the recombinant protein CaTro was expressed in Bl21 bacteria. Afterward, purification was performed by Ni-NTA affinity chromatography. The quality of purified recombinant CaTro was also analyzed using sodium dodecyl sulfate-gel electrophoresis and western blotting. Moreover, to evaluate the induction of immune response, the rabbits were immunized with purified recombinant protein combined with Freund’s adjuvant. The findings of this study revealed molecular weight of expressed protein (CaTro) as 38.00 kDa. Furthermore, anti-CaTro antibody was detected in immunized rabbit's sera through dot blotting; while, there was not any response to the control rabbit's sera. The results suggest that CaTro is a potential candidate to develop an anti- Rh. microplus tick.
کلیدواژه‌های انگلیسی مقاله Cathepsin,Epitope,Tropomyosin,Vaccine
نویسندگان مقاله Zahra Asadollahi |
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Sedigheh Nabian |
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Mohammad Taheri |
Rastegar Reference Laboratory, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Elaheh Ebrahimzadeh |
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

نشانی اینترنتی https://vrf.iranjournals.ir/article_246159_70176c28f0502049460770944fb50a94.pdf
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