این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Veterinary Research Forum، جلد ۱۲، شماره ۳، صفحات ۳۳۹-۳۴۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Kidney tissue engineering using a well-preserved acellular rat kidney ‎scaffold and mesenchymal stem cells ‎
چکیده انگلیسی مقاله The aim of this study was to acquire an effective method for preparation of rat decellularized kidney scaffolds capable of supporting proliferation and differentiation of human adipose tissue derived mesenchymal stem cells (AD-MSCs) into kidney cells. We compared two detergents, the sodium dodecyl sulfate (SDS) and triton X-100 for decellularization. The efficiency of these methods was assessed by Hematoxylin and Eosin (H&E), 4', 6 diamidino-2-phenylindole and immunohistochemistry (IHC) staining. In the next step, AD-MSCs were seeded into the SDS-treated scaffolds and assessed after three weeks of culture. Proliferation and differentiation of AD-MSCs into kidney-specific cell types were then analyzed by H&E and IHC staining. The histological examinations revealed that SDS was more efficient in removing kidney cells at all-time points compared to triton X-100. Also, in the SDS-treated sections the native extracellular matrix was more preserved than the triton-treated samples. Laminin was completely preserved during decellularization procedure using SDS. Cell attachment in the renal scaffold was observed after recellularization. Furthermore, differentiation of AD-MSCs into epithelial and endothelial cells was confirmed by expression of Na-K ATPase and vascular endothelial growth factor receptor 2 (VEGFR-2) in seeded rat renal scaffolds, respectively. Our findings illustrated that SDS was more effective for decellularization of rat kidney compared to triton X-100. We presented an optimized method for decellularization and recellularization of rat kidneys to create functional renal natural scaffolds. These natural scaffolds supported the growth of AD-MSCs and could also induce differentiation of these cells into epithelial and endothelial cells.
کلیدواژه‌های انگلیسی مقاله Decellularization,‎ Extracellular matrix scaffold,‎ Kidney,‎ Mesenchymal stem cells,‎ Recellularization
نویسندگان مقاله Samira Shahraki |
Department of Physiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran‎

Maryam Moghaddam Matin |
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran;

Alireza Ebrahimzadeh Bideskan |
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mohammad Aslzare |
Urology and Nephrology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Ahmad Reza Bahrami |
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

Sara Hoseinian |
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran‎

Sonia Iranpour |
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

Zahra Samadi Noshahr |
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran‎

Abolfazl Khajavi Rad |
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran‎

نشانی اینترنتی https://vrf.iranjournals.ir/article_244405_9a0f2769f11b51afec3a1b886c4f09a0.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات