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Veterinary Research Forum، جلد ۱۲، شماره ۲، صفحات ۱۳۷-۱۴۱
عنوان فارسی
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عنوان انگلیسی Viability of in vitro preserved sperm of Harris’s hawk (Parabuteo unicinctus)
چکیده انگلیسی مقاله Harris hawks (Parabuteo unicinctus) are the raptors with more anthropogenic use, which makes it necessary to improve their reproduction either natural or assisted for sustainable use even though they are not endangered. The aim of this study was to determine fresh and thawed P. unicinctus sperm capacitation and acrosome reaction parameters as indicators of sperm viability in ejaculates of birds with injuries. A total of 30 ejaculates were analyzed as fresh and thawed samples. Basic sperm evaluations as well as in vitro acrosomal reaction capability through the presence and distribution of Ca2+ and N-acetylglucosamine and sialic acid using chlortetracycline and agglutinin-fluorescein isothiocyanate Triticum vulgaris were performed,respectively. Tagged samples were observed using epifluorescence microscopy. The results indicated 30.00% decrease in sperm viability following freeze-thaw, while rate of capacitated sperms (10.00%) and the percentage of sperm with acrosomal reaction (40.00%) were increased. The results of the experiment demonstrated that the post-thaw viability of the obtained sperm may be an alternative for use in artificial insemination.
کلیدواژه‌های انگلیسی مقاله Acrosome,Cryopreservation,glucosamine,Membrane
نویسندگان مقاله Cuauhtémoc Cruz-Valencia |
Department of Animal Reproduction, University Autonomous Metropolitan, Iztapalapa, Mexico

José Antonio Herrera-Barragán |
Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico

Fernando Gual-Sill |
Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico

Juán José Pérez-Rivero |
Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico

Zulma América Soto-Guerrero |
Secretary of Environment, Bird of Prey Rehabilitation Center, Aguascalientes, Mexico

Fernanda Rodriguez-Hernández |
Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico

Juán Gabriel Rivera-Martínez |
Department of Animal Reproduction, University Autonomous Metropolitan, Iztapalapa, Mexico

نشانی اینترنتی https://vrf.iranjournals.ir/article_243068_27837ed217f99f36779e0235de5c993b.pdf
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