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JCR 2016
جستجوی مقالات
شنبه 22 آذر 1404
Veterinary Research Forum
، جلد ۱۱، شماره ۲، صفحات ۱۲۱-۱۲۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژههای فارسی مقاله
عنوان انگلیسی
Some probiotic properties of <i>Lactobacillus</i> species isolated from honey and their antimicrobial activity against foodborne pathogens
چکیده انگلیسی مقاله
Lactobacilli commonly used as a probiotic and they can be isolated from various sources such as fermented foods and gastrointestinal tracts of humans and animals. The aims of this study were isolation and identification of lactobacilli from honey and investigation of some probiotic properties and antimicrobial effects against foodborne bacterial pathogens. A total of 88 honey samples were collected from different areas in Iran. About 1.00 g of each honey was cultured in de Man, Rogosa, and Sharpe (MRS) broth and then sub-cultured on MRS agar. The isolates were assessed for probiotic potentials such as tolerance to acid and bile. Then, antimicrobial activity of isolates against seven foodborne pathogens including
Listeria monocytogenes
,
Shigella flexneri
,
Staphylococcus aureus
,
Salmonella
enteritidis
, Enteropathogenic
Escherichia
coli
,
Escherichia
coli
O157 H7 and
Bacillus
cereus
was investigated. From 88 honey samples, 39 isolates were identified by 16S rDNA gene sequencing method. Fructophilic lactic acid bacteria (FLAB) with 29 (74.00%) isolates were dominant identified bacteria (27
L. kunkeei
and two
Fructobacillus fructosus
). Also, four
L. plantarum,
two L
. paracasei,
one
L. brevis,
one
L. rhamnosus,
one
L. casei
and one
L. fermentum
were identified. Two
L. kunkeei
isolates and one
F. fructosus
isolate were resistant to acid and bile salt. Two
L. rhamnosus
isolates and one
L. paracasei
isolate inhibited all pathogens (100%). This is the first study in Iran that isolated lactobacilli from honey. The FLAB especially
L. kunkeei
were isolated as dominated species from honey. Some lactobacilli isolates have probiotic potential and may be useful for the prevention and treatment of infections, but more investigations are needed.
کلیدواژههای انگلیسی مقاله
Antimicrobial, foodborne pathogens, Honey, Lactobacillus, Probiotic
نویسندگان مقاله
Elahe Lashani |
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Abolfazl Davoodabadi |
Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
Mohammad Mehdi Soltan Dallal |
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی
http://vrf.iranjournals.ir/article_39699_49790e366de1573a9bb824185d7bc940.pdf
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