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Journal of Medicinal Plants and By-products، جلد ۱۲، شماره ۴، صفحات ۵۰۵-۵۱۱
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عنوان انگلیسی Efficiency of ß-carbolines Presented in the Seeds of Peganum harmala L. as Antiproliferative Agent Against Breast Cancer Cell Line
چکیده انگلیسی مقاله Harmaline and harmine, are the major ß-carbolines present in the seeds of the Peganum harmala L. These compounds are known as herbal active principals with potential use in pharmaceutical and medicine. To assess the growth inhibitory effect of phyto-alkaloids, harmaline and harmine, on cancer cell lines. The P. harmala L.’s alkaloids were extracted by acidic/basic extraction method and identified by two methods, Fourier Transform Infra-Red Spectroscopy (FTIR) and High-Performance Liquid Chromatography (HPLC). breast cancer cell lines, MDA_MB_231, were subjected to different concentration (1–100 μg. mL -1) of the P. harmala extract at different time courses (24h, 48h). Methylthiazol Tetrazolium (MTT) test, the half maximal inhibitory concentration (IC50) and the morphological changes through optical microscopy were evaluated cell lines, the P. harmala extract decreased cell viability in longer time exposure in a dose dependent manner. The more concentrated extract led to higher motility of MDA-MB-231 at 24h.It was observed that 30 μg. mL -1 is the minimum lethal dose that kills approximately 50% of cells at 24 hours in MDA-MB-231 cell line (IC50). The morphological observation ensured the apoptosis nature of P. harmala on cells as their membrane kept intact and no membrane permeabilization was observed. The results revealed that the P. harmala extracts decreased significantly growth rate and cell survival of cancer cell lines. higher growth inhibition of MDA-MB-231 cell line by the P. harmala extract was confirmed. 
کلیدواژه‌های انگلیسی مقاله Cell viability, FT-IR, HPLC, Harmine, Harmaline
نویسندگان مقاله Sattar Tahmasebi Enferadi |
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Zahra Salimizadeh |
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Frouzandeh Mahjoubi |
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

نشانی اینترنتی https://jmpb.areeo.ac.ir/article_127466_15d09b9b389c5131d42e2c14296fa54f.pdf
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