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Journal of Livestock Science and Technology، جلد ۹، شماره ۲، صفحات ۲۱-۲۹
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عنوان انگلیسی Association of novel polymorphisms in follicle stimulating hormone beta <i>(FSHβ)</i> gene with litter size in Mehraban sheep
چکیده انگلیسی مقاله Follicle-stimulating hormone (FSH) is necessary for the hypothalamic-pituitary-gonadal axis, and plays an important role in reproduction by binding to a specific receptor (FSHR) through β-subunit of FSH in the surface of the ovarian granulosa cells. This study aimed to characterize FSH β-subunit gene (FSHβ) polymorphism and its association with litter size (LS) using a sample of 118 Mehraban sheep. Polymerase chain reaction (PCR) was performed to amplify two fragments of 300 bp and 431 bp of the ovine FSHβ gene (Oar_v4.0; Chr 15, NC_019472.2). Polymorphisms in the studied fragments were then explored using single strand conformational polymorphism (SSCP) and DNA sequencing methods. A total of seven single-nucleotide polymorphisms (SNPs), including g.59078564 C>G, g.59078624 T>C, g.59078655 T>C, g.59078691 T>C, g.59078754 C>A, g.59080186 G>C and g.59080365 C>T, were found among the six detected SSCP patterns A to F. Moreover, two novel indel polymorphisms called e.g., g.59078702del8-bp−ins64-bp and g.59078726ins54-bp were identified among the three different SSCP genotypes patterns G to I. We found significant differences on prolificacy categories between SSCP genotypes patterns D, E and F (P < 0.01) that simultaneously represented SNP polymorphisms of g.59078754 C>A, g.59080186 G>C and g.59080365 C>T. Similarly, novel indel polymorphisms revealed a significant difference on prolificacy categories between SSCP genotypes patterns G, H and I (P < 0.05). Our results suggest that the FSHβ is a strong candidate gene to associate with the LS in sheep.
کلیدواژه‌های انگلیسی مقاله FSHβ gene, litter size, Mehraban sheep, polymorphism
نویسندگان مقاله Zahra Mokhtari |
Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

Ahmad Ahmadi |
Department of Animal Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan - IRAN

Pouya Zamani |
Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

Reza Talebi |
Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Mohammad Reza Ghafari |
Department of Systems and Synthetic Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

نشانی اینترنتی https://lst.uk.ac.ir/article_3078_65b0671e2f2a7055bebee96d49d3badb.pdf
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