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Middle East Journal of Cancer، جلد ۱۵، شماره ۱، صفحات ۱-۷
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عنوان انگلیسی Evaluation of Fis-1 and miR-484 Expression Levels in Tumor Tissue Samples and Healthy Tumor Margins in Lung Cancer
چکیده انگلیسی مقاله Background: MicroRNAs (miRNAs) regulate gene expression and various cellular activities. They also hold significant importance in the progression and development of human malignancies. Among these, miRNA-484 and the Fis-1 gene have been identified as having substantial roles in lung cancer. This study aims to ascertain miRNA-484 and Fis-1 gene expression levels in non-small cell lung cancer (NSCLC) patients.
Method: In this case-control study, 45 pairs of tumor tissues and their corresponding healthy margin tissues were surgically obtained from NSCLC patients and promptly preserved in liquid nitrogen after excision. Total RNA extraction was performed using TRIzol, followed by cDNA synthesis using a designated kit. Afterward, we used quantitative reverse transcription polymerase chain reaction (qRT-PCR) to measure the expression levels of miRNA-484 and the Fis-1 gene. Furthermore, the clinicopathological characteristics of the NSCLC patients were assessed.
Results: Our findings revealed an upregulation of miRNA-484 expression and downregulation of Fis-1 gene expression in NSCLC tissues compared with non-tumor tissues. Additionally, significant correlations were observed between miRNA-484 and Fis-1 gene expression levels and clinicopathological features of the patients, including factors such as lymph node involvement and distant metastasis.
Conclusion: These findings suggest the potential utility of Fis-1 and miR-484 as prognostic and diagnostic markers in NSCLC.
کلیدواژه‌های انگلیسی مقاله miRNA-486, Fis-1 Protein, Carcinoma, Non-small-cell lung, Malignancy
نویسندگان مقاله Leila Namvar |
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Mehdi Nadiri |
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Zahra Mohebbi |
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Milad Asadi |
Department of Basic Oncology, Ege University, Institute of Health Sciences, Izmir, Turkey

Maryam Seyyedi |
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Amir Amiri-Sadeghan |
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Zahra Soleimani |
Rahat Breathe and Sleep Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Shahriyar Hashemzadeh |
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Yeganeh Khalili |
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Habib Zarredar |
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

نشانی اینترنتی https://mejc.sums.ac.ir/article_49487_26a1e6f05bcd0022eb9a2a77c93b83d5.pdf
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