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Cell Journal، جلد ۲۶، شماره ۱، صفحات ۸۱-۹۰
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عنوان انگلیسی Effects of Streptozotocin Induced Diabetes on One-Carbon Cycle and Sperm Function
چکیده انگلیسی مقاله Objective: Diabetic men suffer an increased risk of infertility associated with signs of oxidative damage and decreased
methylation in sperm pointing to a deficit of the one-carbon cycle (1CC). We aimed to investigate this deficit using mice
models (type 1 and 2) of streptozotocin-induced diabetes.
Materials and Methods: In this experimental study, 50 male mice, aged eight weeks, were divided randomly into
four groups: sham, control, type 1 diabetes mellitus (DM1), and DM2. The DM1 group was fed a normal diet (ND) for
eight weeks, followed by five consecutive days of intraperitoneal administration of Streptozotocin (STZ, 50 mg/kg body
weight). The DM2 group was fed a high-fat diet (HFD) for eight weeks, followed by a single intraperitoneal injection
of STZ (100 mg/kg). After twelve weeks, all the mice were euthanized, and study parameters assessed. In the sham
group, citrate buffer as an STZ solvent was injected.
Results: Both types of diabetic animals had serious impairment of spermatogenesis backed by increased DNA damage
(P=0.000) and decreased chromatin methylation (percent: P=0.019; intensity: P=0.001) and maturation (P=0.000).
The 1CC was deeply disturbed with increased homocysteine (P=0.000) and decreased availability of carbon units
[methionine (P=0.000), serine (P=0.088), folate (P=0.016), B12 (P=0.025)] to feed methylations.
Conclusion: We have observed a distinct impairment of 1CC within the testes of individuals with diabetes. We
speculate that this impairment may be linked to inadequate intracellular glucose and diminished carbon unit supply
associated with diabetes. As a result, interventions focusing on enhancing glucose uptake into sperm cells and providing
supplementary methyl donors have the potential to improve fertility issues in diabetic patients. However, additional
clinical testing is required to validate these hypotheses.
کلیدواژه‌های انگلیسی مقاله Diabetes, Glucose, Methylations, One-carbon cycle, Spermatogenesis
نویسندگان مقاله Farnaz Pouriayevali |
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, Isfahan, Iran

Marziyeh Tavalaee |
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, Isfahan, Iran

Fatemeh Kazeminasab |
Department of Physical Education and Sport Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran

Maurizio. Dattilo |
Parthenogen, R&D Department, Lugano, Switzerland

Mohammad Hossein Nasr-Esfahani |
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, Isfahan, Iran

نشانی اینترنتی https://www.celljournal.org/article_709479_0066c4e51840f1b284aa65270e6fc97d.pdf
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