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Iranian Journal of Microbiology، جلد ۱۶، شماره ۱، صفحات ۶۸-۷۸
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عنوان انگلیسی Investigating the therapeutic potential of Bifidobacterium breve and Lactobacillus rhamnosus postbiotics through apoptosis induction in colorectal HT-29 cancer cells
چکیده انگلیسی مقاله Background and Objectives: Colorectal cancer (CRC) is a prevalent form of cancer worldwide. Recent studies suggest that postbiotics derived from probiotic bacteria have the potential as an adjunct therapy for CRC. This study investigates the anti-cancer effects of Bifidobacterium breve (B. breve) and Lactobacillus rhamnosus (L. rhamnosus) postbiotics on the HT-29 cell line. Materials and Methods: Through MTT and scratch assay, we investigated the anti-proliferation and anti-migration effects of B. breve and L. rhamnosus postbiotics on HT-29 cells. Furthermore, postbiotic-mediated apoptosis was assessed by analyzing the expression of Bax, Bcl-2, and caspase-3. We also investigated the effects of B. breve postbiotics on the expression of three important genes involved in metastasis, including RSPO2, NGF, and MMP7. Consequently, we validated the expression of selected genes in twelve adenocarcinoma tissues. Results: The results demonstrated the significant impact of postbiotics on HT-29 cells, highlighting their ability to induce anti-proliferation, anti-migration, and apoptosis-related effects. Notably, these effects were more pronounced using B. breve postbiotics than L. rhamnosus. Additionally, B. breve postbiotics could inhibit metastasis through upregulation of RSPO2 while downregulating NGF and MMP7 expression in HT-29 cells. Conclusion: Our research suggests that postbiotic metabolites may be effective biological products for the prevention and treatment of cancer.
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نویسندگان مقاله | Nafiseh Erfanian
Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran


| Hossein Safarpour
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran


| Tahmineh Tavakoli
Department of Internal Medicine, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran


| Mohammad Ali Mahdiabadi
Department of Internal Medicine, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran


| Saeed Nasseri
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran


| Mohammad Hassan Namaei
Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/4340
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