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Iranian Journal of Toxicology، جلد ۱۸، شماره ۱، صفحات ۴۵-۵۱
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عنوان انگلیسی Toxicity of Antibiofilm Alginate Lyase from Streptomyces olivaceus on Wistar Rats (Rattus norvegicus)
چکیده انگلیسی مقاله Background: The alginate lyase is known to have antibiofilm activity. Toxicity tests for the alginate lyase produced by Streptomyces olivaceus originating from Serena Kecil Island, North Sulawesi, Indonesia have never been reported. This research conducted an in vivo toxicity study of the alginate lyase using Wistar rats for its development as an oral anti-biofilm agent.
Methods: Twenty male Wistar rats were divided equally into a technical control (TC) group which was only given food and drink, a negative control (NC) which was given Phosphate Buffer Saline 1x, an experimental group (E1) which was given alginate lyase at a dose of 0.63 g/kgBW, and (E2) which was given alginate lyase at a dose of 20.85 g/kgBW. Toxicity tests were carried out for 7 days by observing the parameters of changes in body weight and behavior of rats, changes in AST and ALT enzyme levels, and evaluation of macroscopic and microscopic damage of the liver.
Results: After 7 days of treatment, rats in the NC, E1, and E2 groups experienced insignificant weight loss, also had normal behavior and did not experience symptoms of toxicity or death. AST and ALT levels in rats which was given alginate lyase (E1 and E2) decreased significantly but were still within the normal range. Alginate lyase also causes an adaptive stress response and parenchyma degeneration of liver cells.
Conclusion: Alginate lyase at a dose of 0.63 g/kgBW and a dose of 20.85 g/kgBW were considered safe for rats (Rattus norvegicus) and has the prospect of being developed as an anti-biofilm agent.
 
کلیدواژه‌های انگلیسی مقاله Alginate lyase, biofilm, in vivo toxicity, liver, Streptomyces olivaceus
نویسندگان مقاله | Maya Dian Rakhmawatie
Department of Biomedical Sciences, Faculty of Medicine, Universitas Muhammadiyah Semarang


| Resi Tondho Jimat
Magister Study Program of Clinical Laboratory Science, Postgraduate Program, Universitas Muhammadiyah Semarang


| Stalis Norma Ethica
Magister Study Program of Clinical Laboratory Science, Postgraduate Program, Universitas Muhammadiyah Semarang


| Nanik Rahmani
Research Center for applied Microbiology, Organization Research of Life Sciences and Environment, National Research and Innovation Agency


| Siti Eka Yulianti
Research Center for applied Microbiology, Organization Research of Life Sciences and Environment, National Research and Innovation Agency


| Rike Rachmayati
Research Center for applied Microbiology, Organization Research of Life Sciences and Environment, National Research and Innovation Agency


| Nuryati Nuryati
Research Center for applied Microbiology, Organization Research of Life Sciences and Environment, National Research and Innovation Agency


| Akhirta Atikana
Research Center for applied Microbiology, Organization Research of Life Sciences and Environment, National Research and Innovation Agency


| Shanti Ratnakomala
Research Center for Biosystematics and Evolution, Organization Research of Life Sciences and Environment, National Research and Innovation Agency


| Puspita Lisdiyanti
Research Center for Biosystematics and Evolution, Organization Research of Life Sciences and Environment, National Research and Innovation Agency


| Yopi Yopi
Deputy for Regional Research and Innovation, National Research and Innovation Agency


| Dewi Seswita Zilda
Research Center for Deep Sea, Earth Sciences and Maritime Research Organization, National Research and Innovation Agency
نشانی اینترنتی http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1151-1&slc_lang=en&sid=1
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