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Acta Medica Iranica، جلد ۲۰۱۰، شماره ۱۰، صفحات ۲۷۹-۲۸۲
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عنوان انگلیسی Result Variation and Efficiency Kinetics in Real-Time PCR
چکیده انگلیسی مقاله Fluorescent monitoring of DNA amplification is the basis of real-time PCR. Absolute quantification can be achieved using a standard curve method. The standard curve is constructed by amplifying known amounts of standards under identical conditions to that of the samples.The objective of the current study is to propose a mathematical model to assess the acceptability of PCR resulys.Four commercial standards for HCV-RNA (hepatitis C virus RNA) along with 6 patient samples were measured by real-time PCR, using two different RT-PCR reagents. The standard deviation of regression (Sy,x) was calculated for each group of standard and compared by F-Test. The efficiency kinetics was computed by logistic regression, c2 goodness of fit test was preformed to assess the appropriateness of the efficiency curves.Calculated efficiencies were not significantly different from the value predicted by logistic regression model. Reactions with more variation showed less stable efficiency curves, with wider range of amplification efficiencies.Amplification efficiency kinetics can be computed by fitting a logistic regression curve to the gathered fluorescent data of each reaction. This model can be employed to assess the acceptability of PCR results calculated by standard curve method.
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نویسندگان مقاله رضا شاه سیاه | reza shahsiah
department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

علیرضا | alireza abdollahi
department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

فرید آزموده اردلان | farid azmoudeh ardalan
department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد تقی حقی آشتیانی | mohammad taghi haghi ashtiani
pediatrics center of excellence, children amp;amp; 039;s medical center, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

عیسی جهانزاد | issa jahanzad
department of pathology, imam hospitals complex, school of medicine, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محسن نصیری طوسی | mohsen nassiri toosi
department of gastroenterology, school of medicine, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://acta.tums.ac.ir/index.php/acta/article/view/4048
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