این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Basic Medical Sciences، جلد ۲۷، شماره ۵، صفحات ۵۹۶-۶۰۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Bone marrow stromal cell-conditioned medium regenerates injured sciatic nerve by increasing expression of MPZ and NGF and decreasing apoptosis
چکیده انگلیسی مقاله Objective(s): Despite the many benefits of mesenchymal stem cell (MSC) transplantation for tissue regeneration, there are some limitations to using them, including the high costs, applying invasive procedures, the possibility of transplant rejection, and cell malignancy. This study aimed to investigate the effect of secretions of bone marrow stromal cells (BMSCs) with the cell-free strategy on damaged sciatic nerve with an emphasis on the role of apoptosis and the expression of myelin protein zero (MPZ) and nerve growth factor (NGF) proteins.Materials and Methods: BMSCs were cultured and a 25-fold concentrated conditioned medium (CM) from the cells was provided. After creating a crush injury in the left sciatic nerve of male rats, BMSCs or CM were injected into the injured site of the nerve. Four weeks later, the expression of MPZ, NGF, Bax, and Bcl-2 proteins in the sciatic nerve and histological parameters of the sciatic nerve and gastrocnemius muscle were assessed.Results: The results demonstrated that injection of CM decreased apoptosis and increased expression of MPZ and NGF proteins, improving remyelination and regeneration of the sciatic nerve almost as much as the transplantation of the BMSCs themselves compared to the control group.Conclusion: The results suggest that BMSC secretions may improve remyelination and regeneration of damaged sciatic nerve by increasing the expression of MPZ and NGF and decreasing apoptosis.
کلیدواژه‌های انگلیسی مقاله Apoptosis, Mesenchymal stem cells, Conditioned medium, Myelin protein zero, Nerve growth factor, Sciatic nerve
نویسندگان مقاله | Mitra Ghasemi
Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran|Department of Anatomy, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran


| Athar Talebi
Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran


| Ali Ghanbari
Laboratory of Learning and Memory, Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran


| Parisa Hayat
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran


| Behpour Yousefi
Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran|Department of Anatomy, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran


| Moslem Mohammadi
Department of Physiology, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran


| Mahmood Abedinzade
Medical Biotechnology Research Center, Department of Physiology, School of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran


| Nooshin Ahmadirad
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran


| Sam Zarbakhsh
Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran|Department of Anatomy, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_23764.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات