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Iranian Journal of Basic Medical Sciences، جلد ۲۷، شماره ۵، صفحات ۶۳۰-۶۳۹
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عنوان انگلیسی Genista cephalantha Spach. protects against acetaminophen-induced liver failure via preserving the glutathione redox system, reducing inflammatory response, and inhibiting hepatocyte death in rats
چکیده انگلیسی مقاله Objective(s): The current study was conducted to assess the protective mechanisms of n-BuOH fraction from the aerial part of Genista cephontala (BEGC) on APAP-induced liver injury compared to necrostatine-1 (Nec-1).Materials and Methods: A model of APAP-induced hepatotoxicity was created in male rats by injecting a single dose; 1000 mg/kg APAP, the protective effect was performed with (200 mg/kg; 10 days) BEGC compared to Nec-1, (1.8 mg/kg).Results: BEGC or NeC-1 pretreatment significantly abolished impaired effects in APAP-rats, by decreasing the generation of TBARS and ROS in mitochondrial and cytosolic fractions and maintaining liver function activities. A marked response was observed in the levels of both GSH and GSH-system enzymes in liver homogenates and mitochondrial fractions to BEGC. BEGC/ Nec-1 successfully regulated the inflammatory mediators (IL-β, TNF-α, HMGB1, and acHMGB1) and MPO levels. During APAP treatment, no caspase-3 or -8 activity was detected, and the level of fk18; M30 was higher than the levels of cck18; M65. Moreover, RIPK3 and MLKL levels were increased in the APAP group. These results suggested that necroptosis predominates during the APAP liver injury model. Interestingly, these necroptotic factors were significantly down-regulated by BEGC treatment. Both biochemical and histopathological findings were consistent with each other.Conclusion: From all these findings, the hepatoprotective effect of BEGC could be due to the abundance of polyphenols identified by LC-MS/MS analysis, as well as the synergistic interactions of all contents.
کلیدواژه‌های انگلیسی مقاله Acetaminophen, Cell death, Hepatoprotection Inflammation, LC-MS/MS, Necroptosis, Necrostatin-1, Oxidative stress
نویسندگان مقاله | Boulkandoul Ramzi
Laboratoire de Biologie et Environnement. Université Frères Mentouri Constantine 1, Algérie


| Ameddah Souad
Laboratoire de Biologie et Environnement. Université Frères Mentouri Constantine 1, Algérie


| Chebbah Kawthar
Unité de Recherche, Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques. Frères Mentouri Constantine 1, Algérie


| Erenler Ramazan
Research Laboratory Practice and Research Center, Igdir, University Igdir, Turkiye


| Mekkiou Ratiba
Unité de Recherche, Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques. Frères Mentouri Constantine 1, Algérie


| Benayache Samir
Unité de Recherche, Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques. Frères Mentouri Constantine 1, Algérie


| Benayache Fadila
Unité de Recherche, Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques. Frères Mentouri Constantine 1, Algérie


| Ahmed Menad
Laboratoire de Biologie et Environnement. Université Frères Mentouri Constantine 1, Algérie
نشانی اینترنتی https://ijbms.mums.ac.ir/article_23766.html
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