The aim of this research was to produce a film based on alfalfa seed mucilage with avondol nanoparticles and milk thistle oil. A composite film of alfalfa seed mucilage with avondol nanoparticles (0, 2, 4%) and milk thistle seed oil (0, 1, 2%) was prepared. . The physicochemical properties of the prepared films were investigated. According to the obtained results, adding Avondol nanoparticles and milk thistle seed oil increases the thickness and antioxidant. But increasing the amount of Avondol nanoparticles and milk thistle essential oil in the film decreased the humidity, solubility and water vapor permeability of the films. X-ray diffraction analysis showed that the Avondol nanoparticles were physically incorporated with alfalfa seed mucilage polymer. The results of scanning electron microscope (SEM) showed that the surface morphology of nanocomposite film is heterogeneous compared to alfalfa seed mucilage. Finally, based on the results, adding avondol nanoparticles and milk thistle oil to edible films based on alfalfa seed mucilage improved the thickness, antioxidant and SEM, but weakened the moisture, solubility and permeability to water vapor.