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Iranian Journal of Public Health، جلد ۵۳، شماره ۴، صفحات ۸۵۵-۸۶۶
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عنوان انگلیسی The Role and Function of Secretory Protein Matrix Metalloproteinase-3 (MMP3) in Cervical Cancer
چکیده انگلیسی مقاله Background: We started with RNA-seq analysis and aimed to investigate the possibility of secretory protein matrix metalloproteinase-3(MMP3) as a new diagnosis and therapeutic target in cervical cancer. Methods: The study was conducted on Nov 2021 at the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China. Through conjoint analysis of gene expression data as well as survival rate data, we explored the potential secretary proteins associated with cervical cancer carcinogenesis. One hundred patients aged 38-72 years with clinical stage I-IV cervical cancer, and 100 age-matched healthy women were included. The expression changes in serum of clinical patients was detected. We knockdown or overexpressed the secretory proteins then explored its influence on biological function of cervical cancer cells. Results: By cross-analysis of The Cancer Genome Atlas (TCGA) database and MetazSecKB database, MMP3 gene was most significantly upregulated in cervical cancer patients (P < 0.05). Furthermore, MMP3 protein was remarkably increased in the serum of clinical cervical cancer patients and decreased after receiving treatment. Overexpression of MMP3 in HT-3 cells or culturing new cells using the supernatant of the medium after MMP3 overexpression could increase cell viability (P < 0.05) as well as proliferation (P < 0.05). Knockdown of MMP3 reduced the phosphorylation of PI3K as well as AKT proteins, while the PI3K phosphorylation inhibitors could suppress the impact of MMP3 on increasing cell proliferation as well as viability. Conclusion: MMP3 could be an underlying target for early diagnosis and treat cervical cancer in the future.
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نویسندگان مقاله | Lei Shao
Department of Gynecology, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China


| Wanqiu Liu
Department of Gynecology, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China


| Chunyan Zhang
Department of Gynecology, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China


| Wei Ma
Qiqihar City Hospital of Traditional Chinese Medicine South Department of Gynecology, Qiqihar, China


| Xiao Yu
Department of Obstetrics and Gynaecology, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China


| Jing Han
Department of Gynecology, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China


| Xiaojuan Wang
Department of Gynecology, the Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China
نشانی اینترنتی https://ijph.tums.ac.ir/index.php/ijph/article/view/32103
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