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Iranian Journal of Basic Medical Sciences، جلد ۲۷، شماره ۷، صفحات ۸۰۱-۸۱۲
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چکیده فارسی مقاله
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عنوان انگلیسی Royal jelly induces ROS-mediated apoptosis in acute lymphoblastic leukemia (ALL)-derived Nalm-6 cells: Shedding light on novel therapeutic approaches for ALL
چکیده انگلیسی مقاله Objective(s): Until recently, a conventional chemotherapy regimen for Acute lymphoblastic leukemia (ALL) is considered an efficient therapeutic method in children. However, suboptimal long-term survival rates in adults, disease relapse, and drug-induced toxicities require novel therapeutic agents for ALL treatments. Today, natural products with pharmacological benefits play a significant role in treating different cancers. Among the most valued natural products, honey bees’ royal jelly (RJ) is one of the most appreciated which has revealed anti-tumor activity against different human cancers. This study aimed to evaluate anti-leukemic properties and the molecular mechanisms of RJ cytotoxicity on ALL-derived Nalm-6 cells.Materials and Methods: The metabolic activity was measured by MTT assay. Apoptosis, cell distribution in the cell cycle, and intracellular reactive oxygen species (ROS) level were investigated using flow cytometry analysis. Moreover, quantitative real-time PCR (qRT-PCR) was performed to scrutinize the expression of various regulatory genes. Results: RJ significantly decreased the viability of Nalm-6 cells but had no cytotoxic effect on normal cells. In addition, RJ induced ROS-mediated apoptosis by up-regulating pro-apoptotic genes while decreasing anti-apoptotic gene expression. The results outlined that ROS-dependent up-regulation of FOXO4 and Sirt1 inhibits the cells’ transition to the S phase of the cell cycle through p21 up-regulation. The qRT-PCR analysis of autophagy-related gene expression also demonstrated that RJ induced BECN1 mediated autophagy in Naml-6 cells.Conclusion: Taken together, this study showed that RJ can be utilized as a potent natural substance to induce ALL cells’ programmed cell death. However, further studies are required to examine this compound’s pharmaceutical application.  
کلیدواژه‌های انگلیسی مقاله Acute lymphoblastic - leukemia (ALL), Apoptosis, Nalm-6, Reactive oxygen species - (ROS), Royal jelly (RJ)
نویسندگان مقاله | Somayeh Yazdanparast
Laboratory Hematology and Blood Bank Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Davood Bashash
Laboratory Hematology and Blood Bank Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Amirsalar Nikkhah Bahrami
Laboratory Hematology and Blood Bank Department, School of Allied Medical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran


| Mohammad Ghorbani
Laboratory Hematology and Transfusion Medicine, Department of Pathology, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran|Laboratory Hematology and Transfusion Medicine, Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Gonabad University of Medical Sciences, Gonabad, Iran


| Mehrdad Izadirad
Laboratory Hematology and Blood Bank Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Mehdi Bakhtiyaridovvombaygi
Laboratory Hematology and Blood Bank Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Ahmad Gharehbaghian
Laboratory Hematology and Blood Bank Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran|Pediatric Congenital Hematologic Disorders Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
نشانی اینترنتی https://ijbms.mums.ac.ir/article_23960.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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