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Iranian Journal of Microbiology، جلد ۳، شماره ۲، صفحات ۶۸-۷۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Isolation and genetic characterization of metallo-β-lactamase and carbapenamase producing strains of Acinetobacter baumannii from patients at Tehran hospitals
چکیده انگلیسی مقاله Background and Objective : Carbapenems are therapeutic choice against infections caused by gram-negative bacilli including strains of Acinetobacter baumannii. Resistance to these antibiotics is mediated by efflux pumps, porins, PBPs and ß-lactamases. The aim of this study was to determine the possibility of existence of MBLs, OXAs and GES-1 betalactamase genes among clinical isolates of Acinetobacter collected from Tehran hospitals. Material and Methods: Two hundred and three Acinetobacter isolates were collected from patient at Tehran hospitals. The isolates were identified using biochemical tests. The susceptibility to different antibiotics was evaluated by disk diffusion method and MICs of imipenem were determined using Micro broth dilution method (CLSI). PCR was performed for detection of blaVIM-2, blaSPM-1, blaIMP-2, blaGES-1, blaOXA-51, blaOXA-23 betalactamase genes. Clonal relatedness was estimated by PFGE with the restriction enzyme SmaI. Results and Conclusion: Of 100 isolates of imipenem resistant Acinetobacter spp. collected from Tehran hospitals in 2009 and 2010, 6 isolates produced metallo-beta-lactamases and 94 isolates produced OXA- type carbapenemase. The blaSPM-1, blaGES-1, blaOXA-51, blaOXA-23 genes were detected by PCR among 6, 2, 94 and 84 isolates of A. baumannii, respectively. The MICs of isolates to imipenem were 8-128 μg/mL. PFGE analysis of 29 blaOXA-51 and blaOXA-23-positive A. baumannii isolates gave 6 different patterns. This is the first report of SPM-1 and GES-1 beta-lactamase producing A. baumannii. Production of the OXA-23, OXA-51, GES-1 and SPM-1 enzyme presents an emerging threat of carbapenem resistance among A. baumannii in Iran.
کلیدواژه‌های انگلیسی مقاله Acinetobacter,betalactamase,metallo beta lactamase,OXA-type
نویسندگان مقاله f شاهچراغی | f shahcheraghi
department of bacteriology, pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

m عباسعلی پور | m abbasalipour
department of bacteriology, pasteur institute of iran, tehran, iran and faculty of biology science, shahid beheshti university, tehran. iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

mm فیض آبادی | mm feizabadi
department of microbiology, school of medicine, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

gh ابراهیمی پور | gh ebrahimipour
faculty of biology science, shahid beheshti university, tehran. iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

نسرین | n akbari
department of bacteriology, pasteur institute of iran, tehran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/90
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/91/article-91-313456.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Articles
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