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Iranian Journal of Microbiology، جلد ۳، شماره ۲، صفحات ۱۰۴-۱۰۸
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی A simple method for primary screening of antibacterial peptides in plant seeds
چکیده انگلیسی مقاله Background and Objectives: Regarding the importance of finding new antibacterial drugs, screening of plants as a promising resource are now conducted worldwide. In this study, we report the application of a simple previously described method for screening of different plant seeds in order to find the best resources of plant antimicrobial peptides. Materials and Methods: Total water soluble protein of 10 different plant seeds were extracted and subjected to SDS-PAGE and subsequent agar-overlay bioassays. Standard strains of Staphylococcus aureus, Enterococcus faecium and Escherichia coli were included in the bioassays. This method also was used for total proteins precipitated by Ammonium sulphate which ensure the protein nature of the test substances. Molecular size and the amounts of effective peptides were estimated using Tricin-SDS-PAGE and densitometry. Results : Two different plant seeds showed noticeable antibacterial activities against tested Gram positive bacteria and a moderate inhibitory effect on Gram negative ones. Based on the results of Tricin-SDS-PAGE analysis which were carried out in parallel to bioassays, it was concluded that effective antibacterial substances are peptides with molecular weight of slightly larger than 5 kDa. Conclusion : On the basis of results of agar-overlay experiments and by screening of 10 different herbal seeds, we could introduce seeds of M. sativa L. and Onobrychis sativa Lam., as great sources of putative plant antibacterial peptides. The proposed screening method can be used for screening of large number of different plant seeds and even other parts of the plant body, regarding some necessary modification in total water soluble protein extraction steps.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله a علی احمدی | a aliahmadi
1department of biology, faculty of science, university of isfahan. iran and department of biology, medicinal plants and drug research institute, shahid beheshti university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

r روغنیان | r roghanian
department of biology, faculty of science, university of isfahan. iran.

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

گیتی امتیازی | g emtiazi
department of biology, faculty of science, university of isfahan. iran.

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

a قاسمپور | a ghassempour
department of phytochemist- ry, medicinal plants and drug research institute, shahid beheshti university. tehran. iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/96
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/91/article-91-313462.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Articles
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