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Veterinary Research Forum، جلد ۱۵، شماره ۵، صفحات ۲۳۱-۲۳۶
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عنوان انگلیسی Isolation, serological and molecular methods in screening of Burkholderia mallei in East Azerbaijan province, Iran
چکیده انگلیسی مقاله Glanders caused by Burkholderia mallei is one of the most dangerous zoonotic diseases in solipeds. Clinical diagnosis of this disease in its early stages in horses, is difficult. This study investigated serological and molecular identification of B. mallei in East Azerbaijan province. In the third and fourth quarters of 2020, throughout 2021, and in the first and second quarters of 2022, the complement fixation test (CFT) was performed on 350 horses. The malleination was used to confirm the positive CFT cases. Blood samples were taken for culture and for preparing serums to perform the enzyme-linked immunosorbent assay (ELISA). Deep eye discharge, nostril, cutaneous ulcers and lymph fluid swabs were cultured, and polymerase chain reaction (PCR) was carried out. Eleven horses were CFT-positive. Based on the malleination on the 11 horses, six were affected by glanders, five were not affected (false positive), and one horse was CFT-negative despite exhibiting clinical signs. It was positive by malleination, ELISA and PCR. A total number of seven positive cases of glanders were diagnosed. The B. mallei could not be isolated, but the Burkholderia cepacia complex was isolated in one case. Except for three cases, the results of the CFT, mallein and ELISA tests were consistent. The amount of confidence interval was 95.00%. It is suggested that ELISA could be used as a complement to CFT in screening and, if positive results are observed in one of the tests, the entire herd must be examined more accurately using the mallein and western blot confirmatory tests.
کلیدواژه‌های انگلیسی مقاله Burkholderia mallei,Complement fixation test,ELISA,Glanders,Malleination
نویسندگان مقاله Hassan Tizfahm Tikmehdash |
Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran

Alireza Dehnad |
Department of Livestock Bacterial Diseases Research, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

Nader Mosavari |
Reference Laboratory of Bovine Tuberculosis, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization, Karaj, Iran

Behroz Naghili Hokmabadi |
Infectious and Tropical Diseases Research Centre, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Sanaz Mahmazi |
Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran

نشانی اینترنتی https://vrf.iranjournals.ir/article_711944_67a65503b9576c442e1480a3024b7bad.pdf
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