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Iranian Journal of Public Health، جلد ۴۵، شماره ۱۲، صفحات ۱۶۱۰-۱۶۱۷
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عنوان انگلیسی Fibrinogen and Fibronectin Binding Activity and Immunogenic Nature of Choline Binding Protein M
چکیده انگلیسی مقاله Background: Choline-binding proteins (CBPs) are a group of surface-exposed proteins, which play crucial and physiological roles in Streptococcus pneumoniae . The novel member of CBPs, choline-binding protein M (CbpM) may have binding activity to plasma proteins. This study aimed to clone and express CbpM and demonstrate its interaction with plasma proteins and patients’ sera. Methods: The total length of cbp M gene was cloned in p ET21a vector and expressed in BL21 expression host. Verification of recombinant protein was evaluated by Western blot using anti-His tag monoclonal antibody. Binding ability of the recombinant protein to plasma proteins and the interaction with patients’ sera were assessed by Western blot and ELISA methods. Results: The cbp M gene was successfully cloned into p ET21a and expressed in BL21 host. Binding activity to fibronectin and fibrinogen and antibody reaction of CbpM to patients’ sera was demonstrated by Western blot and ELISA methods, respectively. Conclusion: CbpM is one of the pneumococcal surface-exposed proteins, which mediates pneumococcal binding to fibronectin and fibrinogen proteins.
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نویسندگان مقاله داود افشار | davoud afshar


محمد رضا پورمند | mohammad reza pourmand


محمود جدی تهرانی | mahmood jeddi tehrani


علی اکبر صبور یراقی | ali akbar saboor yaraghi


محمد آذرسا | mohammad azarsa


فاضل شکری | fazel shokri


نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/8636
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/86/article-86-313927.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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