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Research in Molecular Medicine، جلد ۴، شماره ۴، صفحات ۰-۰
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عنوان انگلیسی Phenanthrene biodegradation by mixed bacteria with co-metabolism relationship
چکیده انگلیسی مقاله ABSTRACT: Background: Polycyclic aromatic hydrocarbons are a large group of oil contaminants with Carcinogenic, mutagenic and teratogenic effects. The release of these compounds in soil destroys animals, plants and microbial diversity and reduces physical properties of the soil (soil aggregates destruction, reduce pores and increasing soil bulk density). This study focused on isolation and identification of a Phenanthrene (Phe) degrader bacterium for bioremediation of contaminated soils. Methods: Enrichment technique was used for isolation and the most effective isolates, named ZF1 and ZF2. The degradation experiments were conducted in the mineral salt medium (MSM) containing Phe as the sole source of carbon and energy. selected based on its Phe biodegradation abilities. The isolate was identified using morphological and biochemical tests as well as16S rDNA sequencing and Phe degradation were investigated using protein assay (Bradford) and Gas Chromatography (GC), respectively. Results: Biochemical tests and 16s rDNA gene sequence analysis revealed that isolated bacteria are similar to Pseudomonas aeruginosa and Serratiamarcescens with 99% similarity. After 10 days incubation at 30 °C and pH = 7, bacterial growth and Phe-degrading rate were evaluated by protein assay and gas chromatography (GC) respectively. The results showed mixture of ZF1 and ZF2 bacteria could degrade 83% at concentrations of 200 ppm of Phe whereas single strain culture of two bacteria had poor degradation abilities (less than 15%). Conclusion: Results showed that, isolated bacteria have high potential to degrade Phe with the best results achieved when enriched consortium was used and this mixed was shown to be an appropriate candidate for bioremediation applications. 
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نویسندگان مقاله زهرا فتحی | zahra fathi


غلامحسین ابراهیمی پور | gholamhossein ebrahimipour


زیبا نجمی | ziba najmi


نشانی اینترنتی http://rmm.mazums.ac.ir/browse.php?a_code=A-10-844-1&slc_lang=en&sid=en
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زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده بیوتکنولوژی
نوع مقاله منتشر شده پژوهشی
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