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Iranian Journal of Basic Medical Sciences، جلد ۲۷، شماره ۸، صفحات ۱۰۲۳-۱۰۳۲
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عنوان انگلیسی Renoprotective effect of Limonium duriusculum (de Girard) Kuntze via modulation of oxidative stress/ UPR markers and inflammation during cyclosporine-induced nephrotoxicity in rats
چکیده انگلیسی مقاله Objective(s): The present study aimed to explore the mechanisms underlying the potency of the renoprotective effect of the EtOAc fraction of Limonium duriusculum (EALD) (Plumbaginaceae) against cyclosporine A (CsA), in comparison to vitamin E (Vit. E).Materials and Methods: In the in-vivo experiment, a model of CsA-induced nephrotoxicity was established by dosing male Wistar rats with 25 mg/kg, for 14 days. The protective effect of EALD was investigated through pretreatment of rats with a dose of 200 mg/kg for 14 days, compared to the oral administration of Vit. E at 100 mg/kg. Renal function and markers of oxidative stress were then assessed. Furthermore, a complementary in-vitro study was carried out to evaluate CsA-induced endoplasmic reticulum stress (ERS) and inflammation on cell culture (3T3 cells and MCT cells) using western blot and quantitative RT-PCR..Results: Pretreatment of rats with EALD significantly attenuated the elevated levels of renal dysfunction markers (BUN, creatinine) and suppressed malondialdehyde (MDA) levels; It also significantly regulated the changes in superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxydase (GPx), and glutathione S-transferase (GST) levels as compared to Vit. E, demonstrating a more effective recovery in renal tissues.  Treatment of cells with CsA was linked to the expression of ERS and inflammatory markers activating transcription factor (ATF4), inositol-requiring enzyme 1α (IRE1α), binding immunoglobulin protein (BiP), and monocyte chemoattractant protein-1 (MCP1). In contrast, pretreatment of cells with EALD resulted in a significant decrease in both ERS and inflammatory markers.Conclusion: These findings indicate the renoprotective potential of L. duriusculum, as it demonstrated the ability to ameliorate CsA-induced renal dysfunction through its distinctive antioxidant properties.
کلیدواژه‌های انگلیسی مقاله Cyclosporine, ER stress, Inflammatory markers, Limonium duriusculum, Nephrotoxicity, Oxidative stress
نویسندگان مقاله | Azzedine Redouane-Salah
Laboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie, Université Frères Mentouri Constantine 1, Route Aïn El Bey, 25000, Constantine, Algérie


| Ameddah Souad
Laboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie, Université Frères Mentouri Constantine 1, Route Aïn El Bey, 25000, Constantine, Algérie


| Kerkatou Wafa
Unité de Recherche, Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Université Frères Mentouri, Constantine 1, Route Aïn El Bey, 25 000 Constantine, Algérie


| Kamil Wojnicki
Laboratory of Molecular Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland


| Adrian Ramos
Department of Nephrology and Hypertension, IIS-Fundacion Jimenez Diaz UAM, Madrid, Spain


| Alberto Ortiz
Department of Nephrology and Hypertension, IIS-Fundacion Jimenez Diaz UAM, Madrid, Spain


| Bozena Kaminska
Laboratory of Molecular Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland


| Ahmed Menad
Laboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie, Université Frères Mentouri Constantine 1, Route Aïn El Bey, 25000, Constantine, Algérie
نشانی اینترنتی https://ijbms.mums.ac.ir/article_24221.html
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