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Iranian Biomedical Journal، جلد ۲۱، شماره ۲، صفحات ۷۷-۸۳
عنوان فارسی Evaluation of an Albumin-Binding Domain Protein Fused to Recombinant Human IL-۲ and Its Effects on the Bioactivity and Serum Half-Life of the Cytokine
چکیده فارسی مقاله
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عنوان انگلیسی Evaluation of an Albumin-Binding Domain Protein Fused to Recombinant Human IL-2 and Its Effects on the Bioactivity and Serum Half-Life of the Cytokine
چکیده انگلیسی مقاله Background: Cancer immunotherapy is a promising strategy for cancer treatment. In this strategy,   the immune system is triggered to destroy cancer cells. IL-2 is an important factor in passive cancer immunotherapy that helps modulating some important immune functions. One of the IL-2 limitations is low serum half-life; therefore, repetitive high doses of the injections are required to maintain effective concentrations. High-dose IL-2 therapy results in severe side effects; thus, improvement of its serum half-life would provide therapeutic benefits. Methods: We have investigated a strategy that is able to utilize an albumin-binding domain (ABD) from streptococcal protein G. In this strategy, the fusion protein ABD-rIL-2 binds to serum albumin, which results in improvement of the IL-2 serum half-life. PET26b+ plasmid was used as an expression vector, which encoded rIL-2 and ABD-rIL-2 both fused to pelB secretion signal under the control of the strong bacteriophage T7 promoter. The constructs were expressed in E. coli Rosetta (DE3) and secreted into the periplasm. Results: The analysis of in vitro bioactivity proved that the fusion of ABD to rIL-2 does not interfere with its bioactivity. ABD-rIL-2 fusion protein indicated higher serum half-life compared to rIL-2, when it was tested in the BALB/c mice. Conclusion: The current study provides an alternative strategy to extend the half-life and improve pharmacokinetic properties of rIL-2 without reducing its bioactivity in vitro.
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نویسندگان مقاله الهام ادبی | elham adabi
department of medical biotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

فاطمه صایبی | fateme saebi
department of medical biotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

امین مرادی حسن آباد | amin moradi hasan abad
department of medical biotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

لادن تیموری تولابی | ladan teimoori toolabi
molecular medicine department, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

غلامعلی کاردار | gholam ali kardar
department of medical biotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-634&slc_lang=en&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/125/article-125-316092.pdf
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Medical Biotechnology
نوع مقاله منتشر شده مقاله کامل
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