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Iranian Biomedical Journal، جلد ۲۱، شماره ۲، صفحات ۱۰۶-۱۱۳
عنوان فارسی Investigation of MicroRNA-۲۱ Expression Levels in Serum and Stool as a Potential Non-Invasive Biomarker for Diagnosis of Colorectal Cancer
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Investigation of MicroRNA-21 Expression Levels in Serum and Stool as a Potential Non-Invasive Biomarker for Diagnosis of Colorectal Cancer
چکیده انگلیسی مقاله Background: Most cancer studies focus on exploring non-invasive biomarkers for cancer detection. In the present study, we sought to investigate the expression level of microRNA-21 (miR-21), as a potential diagnostic marker, in serum and stool samples from 40 patients with colorectal cancer (CRC) and 40 healthy controls. Methods: Quantitative real-time RT-PCR was applied to determine the relative expression level of miR-21 in serum and stool. At the same time, the sensitivity and specificity of this marker was evaluated by receiver operating characteristic (ROC) curve analysis. Results: miR-21 expression levels of serum and stool were up-regulated 12.1 (P< 0.05, 95% CI: 5.774-34.045) and 10.0 (P< 0.05, 95% CI: 0.351-16.260) times in CRC patients, respectively, when compared to the control group. The sensitivity and specificity of miR-21 was found to be 86.05% and 72.97%, respectively (an area under the ROC curve [AUC] of 0.783). The stool miR-21 level in CRC patients was much higher than that in the healthy controls, showing a sensitivity of 86.05% and a specificity of 81.08% (AUC: 0.829). The expression level of miR-21 in stool was able to significantly distinguish CRC tumor, node, metastasis stages III-IV from stages I-II, with a sensitivity and specificity of 88.1% and 81.6%, respectively (AUC: 0.872). Conclusion: The results of this study indicated that miR-21 expression levels in serum and stool can be considered as a potential diagnostic biomarker for the diagnosis of CRC patients. However, more studies are required to confirm the validity of miR-21 as a valuable non-invasive diagnostic tool for CRC.
کلیدواژه‌های انگلیسی مقاله Serum, Stool, miR-21, Biomarker, Colorectal Cancer (CRC)
نویسندگان مقاله صیاد بسطامی نژاد | saiyad bastaminejad
department of molecular medicine and genetics, research center for molecular medicine, school of medicine, hamadan university of medical sciences, hamadan, iran;

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

مروت طاهری کلانی | morovat taherikalani
razi herbal medicines research center, department of microbiology, school of medicine lorestan university of medical sciences, khorramabad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی لرستان (Lorestan university of medical sciences)

رضا قنبری | reza ghanbari
digestive oncology research center, digestive disease research institute, tehran university of medical science, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

اکبر اکبری | akbar akbari
abadan school of medical sciences, abadan, iran


نوشین شباب | nooshin shabab
department of molecular medicine and genetics, research center for molecular medicine, school of medicine, hamadan university of medical sciences, hamadan, iran;

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

مسعود سعیدی جم | massoud saidijam
department of molecular medicine and genetics, research center for molecular medicine, school of medicine, hamadan university of medical sciences, hamadan, iran;

سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (Hamadan university of medical sciences)

نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-622&slc_lang=en&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/125/article-125-316095.pdf
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Cancer Biology
نوع مقاله منتشر شده مقاله کامل
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