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Medical Laboratory Journal، جلد ۱۸، شماره ۲، صفحات ۰-۰
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Phylogenetic group determination and genetic diversity of Escherichia coli isolated from domestic animals’ stool specimens and human clinical samples
چکیده انگلیسی مقاله Background: Escherichia coli consists of a wide range of strains with huge diversity in their genome, distributed in nature and the alimentary tracts of animals and humans. This study analyzed the phylogenetic group determination and genetic diversity of E. coli strains isolated from domestic animals and human clinical samples.
Methods: Twenty E. coli isolates from domestic animals were analyzed for phylogenetic grouping. Also, 100 clinical samples and 20 animal samples were evaluated by the enterobacterial repetitive intergenic consensus–polymerase chain reaction (ERIC-PCR) technique. The results and the similarity between the strains were determined based on the Dice similarity coefficient in the SAHN program of the NTSYS-pc software.
Results: The frequency of phylogroups among animal samples were A = 5%, B1 = 65%, B2 = 20%, and D = 10%. Based on the ERIC-PCR results, the clinical strains were allocated into 19 clusters. Most strains were in the E7 cluster. Fifty percent of the E. coli isolated from animal specimens belonged to the E4 group, and the lowest number of strains was in the E3 and E5 (1 strain) groups.
Conclusion: The results confirmed the efficiency and usefulness of the ERIC-PCR tool for the identification and classification of bacteria. Also, we demonstrated the most phylogroup among animal samples.
 
کلیدواژه‌های انگلیسی مقاله Escherichia coli,Phylogeny,Animals,Humans
نویسندگان مقاله | aliehsan karshenas
Department of Pathobiology, science and research branch, Islamic Azad University, Tehran, Iran


| ramak yahya raiat
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


| taghi zahraiee salehi
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


| babak asghari
Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran


| maryam adabi
Infectious Ophthalmologic Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
نشانی اینترنتی http://mlj.goums.ac.ir/browse.php?a_code=A-10-1424-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده علوم آزمایشگاهی
نوع مقاله منتشر شده Research Article
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