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Iranian Journal of Biotechnology، جلد ۲۲، شماره ۲، صفحات ۵۲-۶۳
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عنوان انگلیسی Docking-Based Virtual Screening Method to Selection of Natural Compounds with Synergistic Inhibitory Effects Against Cancer Signaling Pathways Using Multi-Target Approach
چکیده انگلیسی مقاله Background: The complexity of the molecular mechanisms in cancer has led to the ineffectiveness of treatments for many years. To date, the one-target-one-drug approach has shown disappointing consequences for completely eradicating cancer. In comparison, a multi-targeting approach has presented improved outcomes, which may result from the synergistic inhibition of various molecular mechanisms simultaneously. In this regard, medicinal plants contain a wide range of natural compounds that could inhibit multiple targets simultaneously.

Objectives: This study aims to introduce a methodology for identifying medicinal plants that contain effective natural compounds with the most possible synergistic effects to inhibit cancer survival and proliferation in a multi-targeted manner.

Materials and Methods: To select targets, the signaling pathways involved in cancer development were defined from the KEGG database, and the protein-protein interactions (PPIs) of genes within these pathways were investigated using the STRING software. Then 14 proteins with the highest degree were identified as targets. Using the NPASS database, natural compounds were initially filtered based on their IC50 against 50 cancer cell lines. Finally, a total of 1,107 natural compounds were docked to the 14 selected targets involved in cancer and 5 targets involved in general drug side effects.

Results: The targets with the highest protein interactions, as identified by PPI analysis on cancer signaling pathways, were selected as hub proteins. Natural compounds with IC50 less than 20000 nM against cancer cell lines were then docked to these selected targets using the NPASS database. Natural compounds with low binding energy to the selected targets were identified as potential synergistic inhibitors of cancer progression if used together. Additionally, plants reported with the widest range of identified natural compounds were introduced as potential sources of synergistic effects against cancer development.

Conclusions: We have proposed a methodology for pre-screening the natural compounds database to identify potential compounds with a high likelihood of producing a synergistic response against multiple molecular mechanisms in cancer. However, further validation methods are necessary to confirm their effectiveness.
کلیدواژه‌های انگلیسی مقاله cancer,Docking,Multi target,natural compounds,Screening
نویسندگان مقاله Negar Sardarpour |
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran

Zahra Goodarzi |
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran

Sajjad Gharaghani |
Laboratory of Bioinformatics and Drug Design (LBD), Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran

نشانی اینترنتی https://www.ijbiotech.com/article_196866_fda713596e7b6490a3ce925019f75671.pdf
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