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Iranian Journal of Basic Medical Sciences، جلد ۲۷، شماره ۹، صفحات ۱۲۰۹-۱۲۱۳

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عنوان انگلیسی Inactivated herpes simplex virus-1 vaccine formulated in aqueous and alcoholic extracts of propolis boosts cellular and IgG responses
چکیده انگلیسی مقاله Objective(s): In this study, the adjuvant activity of aqueous and alcoholic extracts of propolis was examined on the inactivated herpes simplex virus-1 (HSV-1).Materials and Methods: BALB/C mice were administered with inactivated (HSV-1; the KOS strain) plus alcoholic and aqueous extracts, followed by assessment of the cellular and humoral immune responses. Results: Alcoholic and aqueous extracts, as an adjuvant, revealed a significant increase in lymphocyte proliferation and cytotoxic T lymphocyte (CTL) responses versus the HSV-1 group. In addition, HSV-1 plus alcoholic extract showed a remarkable increase in IFN-γ cytokine and IFN-γ/IL-4 ratio. On the other hand, both alcoholic and aqueous extracts in the HSV-1 vaccine suppressed the IL-4 cytokine response as compared with the HSV-1 vaccine. In addition, HSV-1 plus alcoholic extract showed a significant increment in IgG1, IgG2a, and IgG2b isotypes as compared with the HSV-1 vaccine.Conclusion: Propolis extracts seem to modulate the immune response against inactivated HSV-1 model and can be used as a suitable vaccine adjuvant or a component of a complex adjuvant against infectious diseases.
کلیدواژه‌های انگلیسی مقاله Adjuvant, HSV-1, Propolis, Th1, Vaccine potency

نویسندگان مقاله | Sanaz Mojarab
Advanced Therapy Medicinal Product (ATMP) Department, Breast Cancer Research Center, Motamed Cancer Institute, Academic Center for Education, Culture, and Research (ACECR), Tehran, Iran|Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran


| Pegah Karimi
Department of Biochemistry, Faculty of Basic Sciences, Islamic Azad University, Central Tehran Branch, Tehran, Iran


| Delavar Shahbazzadeh
Venom and Biotherapeutics Molecules Laboratory, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran


| Majid Moghbeli
Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran


| Kamran Pooshang Bagheri
Venom and Biotherapeutics Molecules Laboratory, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran


| Arezoo Beig Parikhani
Venom and Biotherapeutics Molecules Laboratory, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran


| Rada Dehghan
Venom and Biotherapeutics Molecules Laboratory, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran


| Ehsan Zafari
Advanced Therapy Medicinal Product (ATMP) Department, Breast Cancer Research Center, Motamed Cancer Institute, Academic Center for Education, Culture, and Research (ACECR), Tehran, Iran


| Amir Moravej
Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran


| Mohammad Hassan Pouriyayevali
COVID-19 National Reference Laboratory, Pasteur Institute of Iran, Tehran, Iran|Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran, Iran


| Seyedeh Faranak Mirtalebi
Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran


| Mahdi Pakjoo
Advanced Therapy Medicinal Product (ATMP) Department, Breast Cancer Research Center, Motamed Cancer Institute, Academic Center for Education, Culture, and Research (ACECR), Tehran, Iran


| Shaghayegh Yazdani
Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran|Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran


| Meghdad Abdollahpour-alitappeh
Cellular and Molecular Biology Research Center, Larestan University of Medical Sciences, Larestan, Iran


| Mehdi Mahdavi
Advanced Therapy Medicinal Product (ATMP) Department, Breast Cancer Research Center, Motamed Cancer Institute, Academic Center for Education, Culture, and Research (ACECR), Tehran, Iran|Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran|Immunotherapy Group, The Institute of Pharmaceutical Science (TIPS), Tehran University of Medical Science, Tehran, Iran



نشانی اینترنتی https://ijbms.mums.ac.ir/article_24223.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Short Communication
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