The aim of the research was to determine the effect of feeding frequency on growth indicators, carcass composition, activity of some digestive enzymes and survival of elephant fish (Huso huso) larvae. For this purpose, the total number of 10800 great sturgeon (Huso huso) larvae were randomly stocked in 36 500-liter fiberglass tanks containing 100 liters of well water, for 31 days with a feeding frequency of 6 and 12 times a day using Artemia nauplii, Artemia biomass, Daphnia, chironomid larvae and formulated diet  were reared. The results showed that, feeding larvae with chironomid larvae or Artemia biomass, can increase the survival rate and weight of larvae, respectively. The survival percentage of the treatments on different days after hatching of the larvae during 12 times of feeding per day in most treatments was significantly higher than that of 6 times of feeding per day (p <0.05). The average weight of larvae in different treatments, when feeding 12 times a day compared to feeding 6 times a day, had no statistically significant difference (p >0.05). In most treatments, carcass protein content of larvae, during 12  and 6 times feeding a day, had no statistically significant difference (p >0.05). But the carcass fat content, during 12 times feeding per day in half of treatments, was significantly higher than 6 times feeding per day (p <0.05). The activity of pepsin, trypsin and lipase enzymes, during 12 times feeding a day was significantly more than 6 times a day feeding. Totally, feeding larvae 12 times a day was preferable to feeding 6 times a day, based on improvement of survival of larvae, condition factore and activity of enzymes.