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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۲، صفحات ۱۸۷-۱۹۲
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چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The miR-383-LDHA axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer
چکیده انگلیسی مقاله Objective(s): To explore the correlation between expression patterns and functions of miR-383 and LDHA in hepatocellular cancer (HCC). Materials and Methods: We detected the expression of miR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate production assay to explore the function of miR-383 in cell proliferation, invasion and glycolysis in HCC cell lines. Luciferase reporter assay was used to explore whether LDHA was a target gene of miR-383. Western blot and qRT-PCR were used to further confirm LDHA was targeted by miR-383. Then the above functional experiments were repeated to see whether the function of LDHA could be inhibited by miR-383. Results: The results of qRT-PCR showed that miR-383 was down-regulated in HCC tissues compared with their matched adjacent normal tissues. Functional experiments showed that overexpression of miR-383 significantly suppressed cell proliferation, invasion and glycolysis. Luciferase reporter assay showed LDHA was a target gene of miR-383 and expression of LDHA was inversely correlated with that of miR-383 in HCC. Besides, increased cell proliferation, invasion and glycolysis triggered by LDHA could be inhibited by overexpression of miR-383 in HCC cell lines. Conclusion: Our study proved that miR-383 is down-regulated in HCC and acts as a tumor suppressor through targeting LDHA. Targeting the miR-383-LDHA axis might be a promising strategy in HCC treatment.
کلیدواژه‌های انگلیسی مقاله Hepatocellular cancer, LDHA, MiR-383
نویسندگان مقاله zhixiong fang | zhixiong fang
department of infectious disease, xiangtan city central hospital, xiangtan, people amp;apos;s republic of china


langqiu he | langqiu he
department of infectious disease, xiangtan city central hospital, xiangtan, people amp;apos;s republic of china


هیو جیا | hui jia
department of infectious disease, xiangtan city central hospital, xiangtan, people amp;apos;s republic of china


qiusheng هوانگ | qiusheng huang
department of infectious disease, xiangtan city central hospital, xiangtan, people amp;apos;s republic of china


دان chen | dan chen
edong healthcare city hospital of traditional chinese medicine, inefections disease hospital, huangshi, people amp;apos;s republic of china


zhiwei زانگ | zhiwei zhang
cancer research institute, university of south china, key laboratory of cancer cellular and molecular pathology of hunan provincial university, hengyang, people amp;apos;s republic of china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_8246.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-321246.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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