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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۲، صفحات ۱۹۹-۲۰۸
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عنوان انگلیسی Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression
چکیده انگلیسی مقاله Objective(s): Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. Materials and Methods: For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg BW-1, IP) nicotine-received and high dose (0.4 mg/kg BW-1, IP) nicotine-received groups. Following 7 weeks, the expression of bcl-2, p53 and caspase-3 at mRNA and protein levels were investigated by using reverse-transcriptase PCR (RT-PCR) and immunohistochemical (IHC) analyses, respectively. Moreover, the serum level of FSH, LH and testosterone were evaluated. Finally, the mRNA damage was analyzed by using special fluorescent staining. Results: Nicotine, at both dose levels, decreased tubular differentiation, spermiogenesis and repopulation indices and enhanced cellular depletion. Animals in nicotine-received groups exhibited a significant (P< 0.05) reduction at mRNA and protein levels of bcl-2. More analyses revealed a remarkable (P< 0.05) enhancement in expression of p53 and caspase-3 in comparison to control-sham animals. Finally, nicotine resulted in a significant (P< 0.05) reduction in serum level of testosterone and elevated mRNA damage. Conclusion: Our data showed that, nicotine by suppressing the testosterone biosynthesis, reducing mRNA and protein levels of bcl-2 and up regulating the p53 and caspase-3 mRNA and protein levels adversely affects the spermatogenesis and results in cellular depletion.
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نویسندگان مقاله مریم مصدق | maryam mosadegh
department of comparative histology amp;amp; embryology, faculty of veterinary medicine, urmia university, urmia, iran

سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

شاپور حسن زاده | shapour hasanzadeh
department of comparative histology amp;amp; embryology, faculty of veterinary medicine, urmia university, urmia, iran

سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

مزدک رضی | mazdak razi
department of comparative histology amp;amp; embryology, faculty of veterinary medicine, urmia university, urmia, iran

سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_8249.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-321248.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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