| چکیده انگلیسی مقاله |
Background: Primary biliary cholangitis (PBC) is a condition affecting the liver and immune system. In this study, the impact of autologous bone marrow-derived mononuclear cell (BM-MNC) transplantation on PBC patients was investigated.
Methods: Sixteen eligible PBC patients participated at the National Scientific Medical Center in Astana, Kazakhstan, between 2017 and 2022, and BM-MNCs were harvested from their anterior iliac crest. After isolating and cultivating the BM-MNCs, they were infused back into the patient’s peripheral veins. Changes in BM-MNC and peripheral blood mononuclear cell (PB-MNC) phenotypes were assessed before and after a 24-hour cultivation period and 72 hours post-transplantation. We monitored liver function parameters over 6-month intervals and conducted flow cytometry analysis to assess CD markers on BM-MNCs before and after cultivation and PB-MNCs before and after transplantation. Statistical analysis included the Friedman test for liver parameters and the Wilcoxon signed-rank test for BM-MNC and PB-MNC comparisons.
Results: Our findings revealed significant reductions in liver function tests after multiple transplantations. Flow cytometry analysis before and after a 24-hour culture and autologous BM-MNC infusion revealed the expansion of specific cell populations, with significant increases in CD3+, CD4+, CD16+, CD20+, CD25+, CD34+, CD105+, CD73+, СD117+, and CD34+populations, while CD4+25+, CD34+105+, and CD4+FOXP3+ populations decreased. Interestingly, a contradictory finding was observed with a decrease in bone marrow CD34+105+ cell lines (P=0.03) alongside an increase in peripheral CD34+105+ population (P=0.03).
Conclusion: In summary, our study shows that BM-MNC transplantation in PBC patients leads to changes in immune cell populations and liver function. These findings suggest potential therapeutic applications of BM-MNC transplantation in managing PBC and offer insights into the dynamics of immune cells associated with this treatment approach. |
| نویسندگان مقاله |
Dana Saipiyeva |
Cell Technologies, Transplantation and Research Department, National Scientific Medical Center, Astana, Kazakhstan
Manarbek Askarov |
Cell Technologies, Transplantation and Research Department, National Scientific Medical Center, Astana, Kazakhstan
Nazanin Jafari |
PerciaVista R&D Co. Shiraz, Iran
Rano Zhankina |
Department of Urology and Andrology, Astana Medical University, Astana, Kazakhstan
Paul R. Heath |
Sheffield Institute for Translational Neuroscience (SITraN), Department of Neuroscience, University of Sheffield, 385 Glossop Road, Sheffield S10 2HQ, UK
Larissa Kozina |
Clinical and Diagnostic Laboratory, National Scientific Medical Center, Astana, Kazakhstan
Alyona Boltanova |
Clinical and Diagnostic Laboratory, National Scientific Medical Center, Astana, Kazakhstan
Ardak Omarbekov |
Surgical Diseases, Angiosurgery and Plastic Surgery Department, Astana Medical University, Astana, Kazakhstan
Nurbek Ilyassov |
Department of Surgery, National Scientific Medical Center, Astana, Kazakhstan
Turlybek Tuganbekov |
Surgical Diseases, Angiosurgery and Plastic Surgery Department, Astana Medical University, Astana, Kazakhstan
Nadiar M. Mussin |
General Surgery, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Asset A. Kaliyev |
General Surgery, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Yerlan Sultangereyev |
General Surgery, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Farhad Rahmanifar |
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mahdi Mahdipour |
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Shabnam Bakhshalizadeh |
Reproductive Development, Murdoch Children’s Research Institute, Melbourne, Victoria, Australia
Reza Shirazi |
Department of Anatomy, School of Biomedical Sciences, Medicine and Health, UNSW Sydney, Sydney, Australia
Nader Tanideh |
PerciaVista R&D Co. Shiraz, Iran
Amin Tamadon |
Department of Natural Sciences, West Kazakhstan Marat Ospanov Medical University, Maresyev St, Aktobe, Kazakhstan
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